FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL9_col_B_n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL9_col_B_n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223907
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4470.19963645620726464TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGA207.893951E-570.0370943
CGGACTG7600.067.733246
GACTAGT2500.067.190568
CCCGGAC1050.066.701994
GGACTGT7650.065.873117
GCGGACT2000.064.784315
AACGGAC1700.063.857354
GACTGTA7350.063.8005688
ACTGTAG7450.062.474459
GACGGAC900.062.25524
TAGTCCG2650.062.0667611
CCGGACT3050.062.000055
ACGGACT4650.061.753145
GGACTAG2900.061.5430837
ACTAGTC2750.061.0823339
CGGACTA3050.060.8519026
CGCGGAC352.954239E-760.0317964
CTAGTCC2750.059.80978410
CTGTAGA7750.059.6045310
TCGGACT4000.058.6560675
Produced by FastQC (version 0.11.5)