FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL9_col_B_n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL9_col_B_n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381534
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT8880.23274465709478054TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG7600.067.231186
GGACTGT7350.067.13727
CCGGACT2750.064.9036255
ACTGTAG7250.063.2360469
GACTGTA7200.063.1891258
GCGGACT2250.060.6615565
TCGGACT3350.060.5919085
TAGTCCG1950.059.22577711
CGGACTC1250.055.995286
CTGTAGA8100.055.7360510
GCCGGAC700.054.995374
CTAGTCC2100.054.99536510
CGGACTA2550.054.8973356
CCCGGAC900.054.439864
CGCGTAG200.00695081152.4955820
TGTAGAA8800.051.70018411
ACCGGAC1300.051.1495364
CACCGCG1650.050.904870
ACGGACT4700.050.6340335
ATCGGAC1950.050.2521744
Produced by FastQC (version 0.11.5)