FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL9_col_B_n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL9_col_B_n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484014
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG17830.36837777419661416TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT6190.12788886271884697TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5620.1161123438578223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT1200.058.3394475
CGGACTG2900.055.523066
GACTGTA2750.053.4491048
GGACTGT2900.053.098047
ACTGTAG2950.049.825439
CCCGGAC651.8553692E-1048.466624
CGGACTC1400.047.504986
GCGGACT1200.046.671565
CGGGGGT1400.045.00006544
CGGACTA1100.044.5501256
TCGGACT1550.040.649425
TCGGGGG5700.039.298343
TAGTCCG1250.039.1960111
ATCGGGG6650.036.83835242
CTGTAGA4000.036.74625810
ACGGACT3300.035.003675
GGGGGTA708.6859836E-735.00005362
GGACTCT1800.033.052197
TCCGGAC751.4882535E-632.670094
AATCGGG7500.032.66333841
Produced by FastQC (version 0.11.5)