FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL8_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL8_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442209
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8300.032.052116
GGACTGT10100.024.2551067
CCGGACT4250.022.2380145
ATCGGGG10750.022.13870442
GACTGTA10650.021.6880668
TCGGGGG10050.020.89716543
ACTGTAG11200.020.3105569
CGGGGGT4400.019.09268844
AATCGGG14200.017.25287241
TCGGACT5600.016.8770645
CAGTCCG6900.016.23056627
GCGGACT4650.015.8083835
CTGTAGA15250.015.14609210
CGGGGGG9150.014.53687444
TAGTCCG6250.013.99866111
CGGACTA6300.013.8905876
GAATCGG18950.013.66683540
ACAGTCC8400.012.91580526
CTAGTCC6800.012.3517610
GTAGAAG21650.011.96190512
Produced by FastQC (version 0.11.5)