FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL7_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL7_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867669
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3800.031.3234236
TCGGGGG7400.027.90539443
ATCGGGG7950.026.413742
CAGTCCG3150.025.55375927
CGGGGGG6700.021.9406744
CGGGGGT2650.021.13243344
AATCGGG10700.020.93347541
GGACTGT6250.019.0386647
GAATCGG13950.015.80561740
GCGGACT2704.39195E-815.5593465
ACAGTCC5650.014.24678726
GACTGTA8150.014.1707838
TCCGGAG19950.014.03410234
CTCCGGA20750.013.66169133
AACGGAC2851.3680328E-613.5120644
CCGGAGA21150.013.23784135
CGGAGAA22200.013.08466636
TCGGACT4701.4370016E-1012.662665
ACTCCGG21850.012.65357332
AGAATCG18900.012.59192839
Produced by FastQC (version 0.11.5)