FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL6_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL6_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251481
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG20570.1643652600399047TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4200.050.0060776
GGACTGT4250.044.4671137
GACTGTA4700.037.2311338
CGGACTA2350.035.7490276
ACTGTAG5300.033.676619
TCGGACT2850.033.1619265
CTGTAGA5550.032.7902310
CCGGACT2850.031.9337065
GCGGACT2050.029.027925
CAGTCCG3500.028.99773227
TAGTCCG3050.028.68628311
TCGGGGG8100.028.51856443
ATCGGGG9350.028.07379342
CTAGTCC3350.026.11736110
TGCGGAC959.389858E-625.792614
GGACTAG3450.025.3603367
ACTAGTC3650.023.9707289
AATCGGG11850.023.6277941
ACAGTCC4400.023.0663826
GACTAGT3800.022.1035358
Produced by FastQC (version 0.11.5)