FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL5_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL5_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7541334
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC4950.014.84757869
ATCGGGG8050.012.60842742
CGTCCCC6805.456968E-1210.80816368
CCTGCCT8101.8189894E-129.93766440
CCGGCAG12400.09.87849436
CGACCGA10800.09.72162828
AGCGACC10850.09.67682826
CGGGTAG11650.09.6157614
CGGGGGG7302.2191671E-109.58934644
GCGACCG11400.09.51696227
GAGCGAC11150.09.10258325
CTAGCCC8504.5474735E-119.0583335
ATGGGGC5552.8643772E-68.82940150
TCCCCCG8001.2605597E-98.74940870
GGCACCC7302.1678716E-88.63069749
AATCGGG13550.08.5235241
GGGGGCG5957.3142655E-68.23582852
GCACCCC6856.6051507E-78.17571350
CGATAGG15700.08.02610556
GGACTGT13700.07.9191727
Produced by FastQC (version 0.11.5)