FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL4_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL4_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7533724
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG6600.016.96910742
AATCGGG12100.010.99120741
CGGGGGG7650.010.98073944
GCGGGGA6603.9080987E-89.01579648
GCACCCC4956.431223E-58.48539850
AGCGGGG6301.7538387E-68.33392847
CGATAGG14250.08.10648756
GGACTGT16500.07.84795147
ATGGGGC6754.69081E-67.778281750
GTCCCCC4500.0020154277.777300469
CCGATAG17200.07.530157655
GGGGGCG4200.0094304457.50063552
CCTGCCT7007.864348E-67.499639540
CTAACGC7502.594963E-67.46645664
TGCGCAT4950.0051169467.07022614
CACTCCG31450.07.01065270
AACGCGT10603.137029E-86.933536569
GGGGCTT8158.941823E-66.87174852
CGTCCCC5650.00243161016.81374168
GCCCTGA7209.17985E-56.80513867
Produced by FastQC (version 0.11.5)