FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL3_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL3_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552008
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG6600.023.33337242
TCGGGGG7000.021.5008843
GGCACTG3450.021.30563560
GGACTGT6200.019.7561637
AATCGGG9300.018.4405341
CCGGACT3501.8189894E-1217.0013625
CGGACTG7550.016.6900796
CGGGGGG7050.016.38364844
ACTGTAG7300.016.29989
CGGGGGT2401.9621802E-716.04232244
GACTGTA7300.015.82039458
TAGGCAC4450.014.94706758
AGGCACT4800.014.58593159
CGCTGGT5050.014.55305517
GAATCGG11600.014.48193240
CAGTCCG5650.014.24641627
GCACTGT4801.8189894E-1213.85446261
CCCGGAC1800.001259247913.6122014
CCCGCTG4901.8189894E-1213.57012215
ATCCCGC5750.013.39001513
Produced by FastQC (version 0.11.5)