FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455614
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG8000.017.93716642
CGGACTG9200.016.3611456
TCGGGGG8000.016.18756143
CCCTGCG3803.6379788E-1215.6605953
CCGACTG3905.456968E-1215.2552718
CGGGGGG7550.014.37107744
GGCACCC2607.8933135E-613.46370657
GACGCCC2901.6698996E-613.2750233
AATCGGG11450.012.83761341
GGACTGT12150.012.673957
CGCGGAC2356.844574E-411.9166784
TCGGACT7100.011.3397265
ACTGTAG12350.011.3351859
GCGGACT4702.4729161E-811.1718865
CGCCTGT4105.7862235E-711.09685635
CGCCCGG3601.8612447E-510.69376635
CGGACTA6603.274181E-1110.6076496
CCCGGGG3451.4238243E-410.14428337
GCCCTGA3451.4239858E-410.1441767
CAGTCCG7603.6379788E-1210.13082227
Produced by FastQC (version 0.11.5)