FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121323
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT14201.170429349752314TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT1640.13517634743618276TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA1270.1046792446609464TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAT150.002216519870.028451
TCTGCGT150.002220149269.9995958
GTAGCCG150.002220149269.999596
ATCGGAC207.900337E-569.999594
CCCGGAC150.002220149269.999594
CGGACTG1400.069.999596
TCGGACT650.069.999585
GGACTGT1450.065.172037
ACTGTAG1350.062.2218559
GACTGTA1400.059.9996458
CACGGAC308.335472E-658.332994
CTAGTCC452.6768248E-854.44412610
CGCGGAA200.006925984352.5213431
GGACTAC200.00693729252.499697
TCAGGCA200.00693729252.4996912
GCGGAAG200.00693729252.499692
TTCTGCG200.00693729252.4996957
CTACAGT1200.052.49968724
CGGACTA608.367351E-1152.4996876
CCGGACT552.2573658E-950.9087945
Produced by FastQC (version 0.11.5)