FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL15_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL15_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958186
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT52660.26892235977583334TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6300.045.0058946
TGCCGTC9750.043.79948850
TATGCCG10750.042.33004448
GCCGTCT8850.041.13427751
ATGCCGT10850.039.35898649
GTATGCC12050.038.3442947
GGACTGT7150.037.6885837
CCGGACT3050.036.726125
CGTATGC12300.036.7102546
TCGGACT4350.036.211645
CCGTCTT10700.035.3317352
GACTGTA7950.033.0156028
ACTGTAG8000.032.8092589
CTCGTAT13700.031.42506844
CAGTCCG4800.031.35106727
TCGTATG14500.029.69126945
CGGACTA3100.029.3586836
CGTCTTC12500.028.00438353
CTGCTTG10400.027.9363559
TCTCGTA16700.025.98943743
Produced by FastQC (version 0.11.5)