FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL13_col_B_n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL13_col_B_n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314962
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT92780.17456380685318162TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20550.037.9866248
TGCCGTC18950.036.94523250
ATGCCGT20500.036.5424449
GCCGTCT16600.035.84945351
GTATGCC23350.033.13164547
CGTATGC23450.031.64656446
CGGACTG7000.026.501756
CCGTCTT23850.024.95205552
TCGTATG29150.024.73716545
CTCGTAT27650.024.5592844
CTGCTTG23450.022.39143859
TATCTCG35500.020.9004341
TCTCGTA33600.020.10549543
CGTCTTC29250.019.3884553
ATCTCGT38050.018.76531642
CGCTCAT53850.017.35229734
CAGTCCG5050.017.32510827
GGACTGT10350.016.5684937
AGGGGGG9400.016.385761
CTTCTGC32900.016.17310756
Produced by FastQC (version 0.11.5)