FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL13_col_B_n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL13_col_B_n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995586
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT52000.17358874023312967TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10700.037.9516448
GCCGTCT8750.036.40801251
ATGCCGT10600.035.9978949
TGCCGTC10250.035.5194250
CGTATGC11750.035.4534346
GTATGCC12850.033.2357847
CGGACTG3500.029.0049326
TCGTATG15200.027.40551845
CTCGTAT15450.026.50670844
CCGTCTT13350.024.91185252
TCTCGTA18950.021.05652843
TATCTCG19900.020.92796741
CGTCTTC15150.020.33484853
CTGCTTG14500.019.7971359
ATCTCGT22950.018.147642
CAGTCCG3750.017.7310227
CTTCTGC16050.017.23120756
TGGGGGG5700.017.197631
GGACTGT5350.017.0071267
GCTTGAA21400.015.04828361
Produced by FastQC (version 0.11.5)