FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL12_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL12_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727522
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT31980.11724928341549583TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT7450.030.54417851
CGGACTG5750.029.2211276
TATGCCG9400.029.04786348
TGCCGTC9000.028.00511750
GTATGCC10650.025.9671947
ATGCCGT10750.025.39952749
CGTATGC11550.024.24685946
CCGTCTT10650.022.02401752
GGACTGT7950.021.1293537
CAGTCCG5250.019.33084327
AGGGGGG4300.018.7274461
CTCGTAT14250.018.42340944
ACTGTAG9000.017.8865839
TCGTATG15550.017.55915845
AGGGCCC1000.0096926917.5003162
CACCGCG3002.910383E-1117.49774670
TGGGGGG4650.017.317851
TATCTCG16450.017.02001841
GACTGTA9250.015.8898458
CTTCTGC12350.015.87538556
Produced by FastQC (version 0.11.5)