FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469452
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT19130.4074964000579399TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5960.12695653655751812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4550.065.404226
GGACTGT4950.060.093427
GACTGTA4850.058.4462288
TCGGACT2400.058.3508265
CCGGACT1600.056.892055
AACGGAC1500.056.0167924
GCGGACT1150.054.7990385
CGGACTC1300.053.8622976
ACTGTAG5700.050.3445179
CGGACTA1450.048.290346
GTCCGCA305.8794895E-446.6607729
CTGTAGA6200.046.28447710
ACGGACT4100.045.2574655
CACCGCG703.8380676E-1044.99431670
TGCGGAC659.977157E-943.089844
ATCGGAC1400.042.5127454
TGCCGTC3900.041.29003550
ATGCCGT4000.040.2577849
CCGGGGT350.001255789739.9949529
CGCCGGT350.001255789739.9949532
Produced by FastQC (version 0.11.5)