FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ColSPL10_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ColSPL10_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246336
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT54390.4363991732566499TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16380.1314252336448598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4450.051.9154666
GCCGTCT7450.046.51673551
GGACTGT4950.045.2499357
TATGCCG9850.044.77439548
TGCCGTC9300.044.41318550
ATGCCGT9750.043.79763449
CCGTCTT8450.041.42605652
GACTGTA5450.038.52998
GTATGCC11600.038.01963847
CGGACTA2450.037.1466876
CGTCTTC9550.036.65593753
CGTATGC12150.036.010546
CTGCTTG8500.035.82722559
ACTGTAG6000.035.5812959
TAGTCCG2400.034.99799311
TCGGACT3150.033.3367735
CTCGTAT12950.033.24400744
TCGTATG13250.033.01962345
CTTCTGC9550.032.9890256
AACGGAC2450.032.860534
Produced by FastQC (version 0.11.5)