FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL9_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL9_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10033897
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20650.035.76687248
TGCCGTC19500.034.8245750
GCCGTCT19600.031.96880151
GTATGCC25600.029.5345447
ATGCCGT24950.029.04145849
CGTATGC34850.022.19759446
CCGTCTT36900.017.64485752
TCGTATG46450.017.25617645
CATCTCG39250.016.1393541
CTCGTAT45950.015.76755844
CGTCTTC44450.014.72729753
ATCTCGT52200.012.80653742
CCATCTC53100.012.45702840
AGATTCC88000.010.69808335
CGAGATT84100.010.6948233
CTTCTGC51050.010.62886856
CTGCGTG8250.010.60792959
GATTCCA86000.010.49928636
CACGAGA89350.010.18382331
GTCACGA89500.010.16670429
Produced by FastQC (version 0.11.5)