FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199524
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT9690.48565586094905877TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG550.063.6197286
CCGGACT252.3893174E-455.9853635
GCCGTCT1300.053.87258551
GTACTGG200.006950841352.4862832
TGCCGTC1450.050.71453550
ATGCCGT1600.050.33719649
ACTGTAG651.8371793E-1048.448879
GGACTGT800.048.1124237
CTGCGTG305.8462395E-446.7012959
TATGCCG1850.045.42769648
CTGCTTG1400.045.03338659
AACGGAC404.5881705E-543.7385674
CTGTAGA757.530616E-1041.9890210
ACTAGTC503.4726272E-641.989029
GACTGTA757.530616E-1041.989028
CCGTCTT1800.040.8533852
CGTATGC2050.039.2875746
GTATGCC2050.039.2875747
CGGACTA556.6834054E-638.1718376
CTTCTGC1850.037.8659156
Produced by FastQC (version 0.11.5)