FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL15m2_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL15m2_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598525
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT15600.2606407418236498TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.040.28306248
GCCGTCT3200.038.28988651
ATGCCGT3500.037.0083549
TGCCGTC3500.037.0083550
GTATGCC4050.036.30448547
CTCGTAT3900.032.3122844
CGTATGC4700.032.02850346
TCGTATG5250.027.339545
CCGTCTT4250.027.18260252
CGTCTTC4600.025.87540453
CATCTCG5000.025.19726241
CTGCTTG4250.024.71145659
ATCTCGT5400.023.33274842
CTTCTGC4650.022.5857456
TCGCGTA800.0026634721.87810743
TCTCGTA5700.021.49428243
GCCGGCT1154.1081134E-521.30915651
GCTTGAA5000.021.00122861
ATGCCGG850.003783723820.5928849
TGCCGGC850.003783723820.5928850
Produced by FastQC (version 0.11.5)