FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL15m1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL15m1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468680
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT16790.35824016386447044TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2950.055.7728348
GCCGTCT2650.054.16076751
GTATGCC3300.051.97912647
TGCCGTC3250.049.54744750
ATGCCGT3600.045.70273649
CGTATGC4000.044.63309546
CCGTCTT3800.036.84879352
TCGTATG4450.036.17861645
CTCGTAT3700.035.94479444
CTGCTTG3000.031.50571659
GCGTATG450.00430655731.11011545
CGTCTTC4350.030.5802653
CATCTCG3850.029.9990441
CTTCTGC3900.029.61759656
TCTCGTA4350.028.16001943
TACCGAG500.00720345328.0020965
ATCTCGT4250.027.99910442
CGCGTAT657.9537526E-426.92221544
GCGTGAA1056.751561E-726.67150761
TGCTTGA3700.026.49129360
Produced by FastQC (version 0.11.5)