FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL14_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL14_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90284
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5950.6590315005981127TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGC150.00221873769.99612417
ACATCCC150.00221873769.99612444
CGGACTG308.320685E-658.33016
TCGGACT252.3798605E-455.9969025
ACGGGGT200.0069329252.49709336
TGGCATC200.0069329252.49709334
CGGGGTC200.0069329252.49709337
GGACTGT305.8520935E-446.664087
CTCTCCT305.8520935E-446.6640829
GCCGTCT1450.043.4458751
TGCCGTC1500.041.99767350
ACGGACT350.001249971939.9977845
GACTGTA350.001249971939.9977848
CCGTCTT1600.039.3728252
CGTCTTC1600.037.20605553
ATGCCGT1700.037.05677449
TATGCCG1750.035.9980148
CTGTAGA601.2046512E-534.99806210
TCTCGTA1850.034.0521743
CATCTCG1750.033.9981241
Produced by FastQC (version 0.11.5)