FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_C24SPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_C24SPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143831
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT17371.2076673317991253TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT1530.10637484269733227TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACT252.817691E-670.044535
CCTATAC150.002221462369.9958330
GCGGACT252.3764989E-456.035635
GCCGTCG252.3846867E-455.99666251
CCCTGAC200.006941371652.49687253
GGACTGT552.2646418E-950.906067
GCCGTCT2100.049.99701751
TATGCCG2450.047.1400548
TGCCGTC2450.047.1400550
CGGACTG604.8621587E-946.6963586
CCGTCTT2250.046.66388752
ACATCCG305.863888E-446.66388734
CGTCTTC2100.046.66388353
GTATGCC2650.044.9029847
TGCTTGA1950.044.8691260
CTGCTTG1800.044.71955559
ATGCCGT2600.044.42043349
CTCGTAT3000.040.830944
CGTATGC2950.040.3365846
TCCTGTA350.001252476239.9976167
Produced by FastQC (version 0.11.5)