FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_ALSPL9_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_ALSPL9_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7109882
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12550.032.35455748
TGCCGTC13050.029.50511250
GCCGTCT12700.027.8376651
GTATGCC16350.025.26267647
ATGCCGT16200.023.76834149
CGTATGC23700.017.87113446
CCGTCTT24800.014.96140352
CATCTCG24050.014.6977641
CTCGTAT33100.012.90097344
TCGTATG34550.012.56242545
CGTCTTC28700.012.31875453
CCATCTC33200.011.38485340
ATCTCGT33900.010.53055942
CGCTGGG4804.4888173E-58.7498463
GATTCCA58450.08.62223336
TCCATCT47150.08.53606939
CTGCTTG32500.08.29343959
TCTCGTA46950.08.27507543
AGATTCC59650.08.2727635
CGAGATT57200.08.2599933
Produced by FastQC (version 0.11.5)