FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_smart_pool2_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_smart_pool2_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16586537
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA3907212.3556514539472584No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC2980551.7969694337039734No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC2225401.3416905530069356No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC2078691.2532392988361585No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA1705711.0283701775723286No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA1650340.9949876818771755No Hit
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC1601820.9657350416183921No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC1470020.8862730056310126No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT1430060.8621811774211819No Hit
TTGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC1106510.6671133341456388No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA971320.5856074718912091No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGAC957390.5772090943395839No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT915710.5520802805311319No Hit
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG864760.521362596664994No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC840040.5064589431778315No Hit
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT827880.4991276961550202No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT815200.49148294185820707No Hit
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC806110.4860025935492141No Hit
TTGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCA730720.44055006780499145No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGT707210.426375921628487No Hit
TTGAGTTAACCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG652780.39356015062095245No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA639300.3854330774410596No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC619330.3733931923221827No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA615100.37084293122789885No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT556800.33569394262346625No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG545140.3286641449025797No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT501680.3024621715792754No Hit
TTGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATA492630.2970059392144364No Hit
TTGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA486830.2935091273121086No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC473110.28523735846729187No Hit
TTGAGTTCATCTGGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGAC429790.25911979094852655No Hit
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG427700.25785973286648084No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG425070.25627410953835633No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC420950.2537901672904959No Hit
TTGAGTTAACCAGGGGAGCATGCAAAGGCCATAGGAAATTGCCCAATATG413180.24910564513858438No Hit
TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC407100.2454400216271787No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA377310.22747967221849866No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGT371910.22422401975771075No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG359820.21693497563716885No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA335690.20238703232627764No Hit
TCAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTT318650.19211364011668017No Hit
TTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGG318440.19198703140987175No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC318400.19196291546571775No Hit
TTGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA317640.19150471252679208No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC316160.19061242259309463No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT315190.1900276109473605No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG314550.18964175584089676No Hit
TTGAGTGCTTTTGAGATCTGCTGCTTGTCCTATTCCCTCAGAATTTTGAT309140.1863800743940703No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA296780.1789282476504891No Hit
TTGAGTTAACCAGGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATG292110.17611271117051136No Hit
TTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAA290770.17530482704135286No Hit
TTGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCT286460.172706334058761No Hit
TTGAGTTAACCAGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATGG284120.17129555132575294No Hit
TTGAGTTAACCAGGGGACCATGCAAAGGCCATAGGAAATTGCCCAATATG265210.1598947387269567No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT263620.15893612994683579No Hit
TTGAGCATATAGGCTGATTTGGTCATTGTCCGTACCCGGGTGGTGAACTC262450.15823073858033174No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAA260270.1569164196239396No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT259090.15620499927139703No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA258490.15584326010908725No Hit
TTGAGTTACACGTGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT256780.15481230349650443No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA250690.15114065099906027No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA250360.1509416944597899No Hit
TCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATA249310.15030865092574777No Hit
TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA246300.14849392612816045No Hit
TTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA230680.13907664993602945No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA229520.1383772875555639No Hit
TTGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA228770.13792511360267667No Hit
TTGAGTGAGTTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA224800.1355316061453937No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT222650.134235374147117No Hit
TTGAGTTAACCAGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGG220840.1331441276741492No Hit
TTGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC217650.13122088112786895No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC211380.12744070688173187No Hit
TTGAGTTCCAATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC208110.12546922844714362No Hit
TTGAGTTAACGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGCT203960.1229671992411677No Hit
TTGAGTTAACCAGATTGCCTCCACGAAAAATACGGTGGATTAAACAAAAG200980.12117056140169584No Hit
TTGAGTTACACGTGCAGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC199830.12047722800726879No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGG199180.12008534391476652No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA196290.11834296694964114No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA194970.11754714079255965No Hit
TTGAGTAGCTAAGACCCCGTAATTGATTACTATTAATAACTAGTCAATAA194890.11749890890425169No Hit
TTGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGC190380.11477983620088993No Hit
TTGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTG188210.11347154623053625No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA188080.11339316941203578No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG185860.11205473451148965No Hit
TTGAGTAGCTAAGGACCCCGTAATTGATTACTATTAATAACTAGTCAATA182690.11014354593728637No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA181920.10967931401232216No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA175200.10562783539445275No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA174760.1053625600087589No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC174590.10526006724610448No Hit
TTGAGTTTCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC169050.1019200089807776No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG168890.10182354520416168No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT168270.1014497480697749No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT168250.1014376900976979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA515200.0136.929243
TTGAGTT4582250.0136.315021
GCCGCCT528050.0134.094741
TTAACGC422550.0133.966356
AGTTAAC1229450.0133.860064
CTTACAC521400.0133.535356
TGAGTTA2440950.0133.303922
GTTAACG430400.0133.185155
TTACACG498900.0132.702476
GAGTTAA1378100.0132.261873
GTTAACC790200.0132.254535
TTAAGGT648000.0132.116621
TTGAGTA3563600.0131.958631
TTATCCG374950.0130.906346
TACACGT504300.0130.424077
TAAGGTC663250.0128.787172
TCCGGCC203050.0127.874669
CAACCCG181800.0127.208828
CAAGACG113950.0126.536242
GTTCGGA235000.0126.329825
Produced by FastQC (version 0.11.2)