FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_smart_pool1_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_smart_pool1_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16224319
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA3936262.4261480558906663No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC2777481.7119239334483005No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC2160421.3315936403863855No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC2118691.3058729922654997No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA1785541.1005330947943024No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA1627131.0028957147600464No Hit
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC1552770.9570632825944805No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC1522240.9382458517981557No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT1391060.8573919188842378No Hit
TTGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC1146770.7068216545791537No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGAC970680.5982870529111268No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT921500.5679745325520289No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA899950.5546920027891464No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC848620.5230543112472086No Hit
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG810500.49955871799611434No Hit
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC779000.48014341927078724No Hit
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT777070.47895384699967997No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT754640.4651289215898677No Hit
TTGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCA740630.4564937363472698No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGT716930.44188603540154753No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC630380.388540190808625No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA614630.37883254144596146No Hit
TTGAGTTAACCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG598380.36881671273845146No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA578930.3568285362239241No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT556100.34275706733823463No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC523800.3228486816611532No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT513320.3163892425931714No Hit
TTGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATA500810.30867859538511294No Hit
TTGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA498840.30746436876641786No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG493490.3041668497765607No Hit
TTGAGTTCATCTGGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGAC441870.27235041421461204No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC419280.2584268714144489No Hit
TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC410280.25287964320721257No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG393400.24247550852519603No Hit
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG391650.24139688081823343No Hit
TTGAGTTAACCAGGGGAGCATGCAAAGGCCATAGGAAATTGCCCAATATG376630.23213917329904574No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG376540.23208370101697337No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGT372200.22940870430370608No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA352990.21756845387470503No Hit
TTGAGTGCTTTTGAGATCTGCTGCTTGTCCTATTCCCTCAGAATTTTGAT334850.20638770724367536No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT331130.2040948529180177No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC317710.19582331930233865No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA312130.19238403781385216No Hit
TTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGG306340.18881532100053014No Hit
TCAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTT298860.18420495800162706No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC297820.18356394496434641No Hit
TTGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCT297690.18348381833468633No Hit
TTGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA297070.18310167594707674No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG282450.1740905119037662No Hit
TTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAA278200.17147098747257128No Hit
TTGAGTTAACCAGGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATG270020.16642917339088314No Hit
TTGAGTTAACCAGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATGG261710.16130723267953495No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT259900.16019162345119076No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA259260.1597971538897873No Hit
TTGAGCATATAGGCTGATTTGGTCATTGTCCGTACCCGGGTGGTGAACTC256520.15810833108002867No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA256420.15804669521105938No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAA256120.1578617876041515No Hit
TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA252230.15546415230124605No Hit
TCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATA250220.15422527133496328No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT249470.15376300231769358No Hit
TTGAGTTAACCAGGGGACCATGCAAAGGCCATAGGAAATTGCCCAATATG246370.1518522903796455No Hit
TTGAGTTACACGTGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT242040.14918345725327514No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA235270.14501070892405407No Hit
TTGAGTGAGTTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA231920.14294590731358278No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA227560.14025858342652164No Hit
TTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA226200.13942033560853925No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA225480.13897655735196035No Hit
TTGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC222330.13703502747942764No Hit
TTGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA214830.13241233730673072No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT206650.1273705232250426No Hit
TTGAGTAGCTAAGACCCCGTAATTGATTACTATTAATAACTAGTCAATAA205840.12687127268639134No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA201790.124375019993135No Hit
TTGAGTTACACGTGCAGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC201390.12412847651725784No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA200280.1234443183716987No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGG199880.12319777489582151No Hit
TTGAGTTAACCAGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGG198810.12253827109785008No Hit
TTGAGTTAACCAGATTGCCTCCACGAAAAATACGGTGGATTAAACAAAAG195390.12043032437910028No Hit
TTGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTG195000.12018994449012005No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG194020.11958591297422098No Hit
TTGAGTAGCTAAGGACCCCGTAATTGATTACTATTAATAACTAGTCAATA191960.11831621407345355No Hit
TTGAGTTCCAATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC191780.11820526950930885No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC189570.11684311680508748No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA184300.11359490651040575No Hit
TTGAGTTAACGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGCT182690.11260256902000017No Hit
TTGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGC179440.11059940327849817No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC175930.108435984277676No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT171540.10573016962992406No Hit
TTGAGTTTCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC171310.10558840713129469No Hit
TTGAGTGAAGTTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA167410.10318460824149228No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA166910.10287642889664583No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT164480.10137867728069203No Hit
TTGAGTTAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGC163850.10099037130618549No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG163070.10050961152822502No Hit
TTGAGTACTGAATGGGAGATGATGCAAAAGATAAATCGCAATTATTCTTC162680.10026923163924477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA505500.0137.804473
TTGAGTT4548100.0136.318281
TTAACGC399350.0134.102686
GCCGCCT522000.0134.015811
AGTTAAC1180200.0133.773074
CTTACAC515450.0133.706056
TGAGTTA2394300.0133.543432
GTTAACG407500.0133.302145
TTACACG493700.0132.669176
TTAAGGT628000.0132.180831
GAGTTAA1330350.0132.034383
TTGAGTA3477400.0131.79581
GTTAACC764900.0131.725315
TTATCCG382150.0131.075976
TAAGGTC637000.0129.744842
TACACGT503000.0129.556997
TCCGGCC221250.0129.332099
CGTCGAT37000.0129.016946
CAACCCG182200.0126.92788
ACTAACG181800.0126.296977
Produced by FastQC (version 0.11.2)