FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_smart_methods_pcr_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_smart_methods_pcr_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919
Sequences flagged as poor quality0
Sequence length150
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG931.8906281764586297No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG230.467574710306973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGG190.38625736938402117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG130.2642813579995934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGT110.2236226875381175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG70.1423053466151657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGG60.12197601138442773No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA60.12197601138442773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT50.10164667615368977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGGTGG50.10164667615368977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGGGGGG50.10164667615368977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT50.10164667615368977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG50.10164667615368977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG100.007022275144.09
TTGAGCA100.007022275144.01
GGAGCGG100.007022275144.08
AAGAGGG304.4326727E-528.80000180-84
GGTAAAA304.4326727E-528.80000165-69
TGGGGTA255.217481E-428.820-24
GGTTAAA353.7792524E-628.865-69
AAAAGAG403.2312346E-728.875-79
ATTAAAA403.2312346E-728.865-69
GAATTAA255.217481E-428.865-69
GTTAAAA502.3756002E-928.865-69
GGGGGCG1050.007235660727.428578
AAAAAAT950.027.2842175-79
AAAAATG750.026.87999775-79
AAAATGG605.620677E-1026.40000275-79
AATGGGG556.5720087E-926.18181880-84
GGGGCGG1100.00908061126.1818189
AAAAAAG2500.025.9275-79
AAAAAGA507.698873E-825.91999875-79
AATTAAA401.0670579E-525.19999965-69
Produced by FastQC (version 0.11.2)