FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_smart_methods_extraction_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_smart_methods_extraction_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844967
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC16635319.687514423640213No Hit
TTGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGC411524.870249370685483No Hit
TTGAGTTGATAACCAAAGATGAGCCTTCCTGTGCTGGGTTCCTTCATGAT331733.925952137775795No Hit
TTGAGATTCGTAAGCCAAAGGCTTTTGTTAGATTGTGAGGCAAGGTAGAG205452.4314559030115968No Hit
TTGAGTGCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG193412.288965131182638No Hit
TTGAGTTCCAGGAAAAGTGAATTCCAGGAATTTATGTTTGGGTAGACCTA120071.4210022403241782No Hit
TTGAGTAAAGCCCATGTACAGTGCTCAGAACAGGGCATGGCCCATAATAA102711.2155504297800979No Hit
TTGAGTGCCTATACCCAGCACAGGAAGGCTCATCTTTGGTTATCAACTCA101981.2069110391293387No Hit
TTGAGTCCTCACCCAACCCAAACCATGCTGGCAGCCTGATCTCCAACTTC95821.1340087837749877No Hit
TTGAGTGCCTATACGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATACC93291.1040667860401647No Hit
TCAAGGAACTGCTCAGAGGAGCCGTTTACAGCACAGAAGCAAGAGAAGAG85411.0108087061388196No Hit
TTGAGTCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGGT81930.9696236657762967No Hit
TTGAGTCCCTTTTCCCTTTGCTGATTCTAATCTGTATCTTTTCCCTGTAA69870.8268961983130702No Hit
TTGAGTGCCTATAGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATACCA67720.8014514176293276No Hit
TTGAGTGCCTATAAGAAGATAAGTTTTGTATTTCCATCTAAATAGATACA59070.6990805558086884No Hit
TTGAGTGCCTATACTGATCAATTTGATAACCCTTGGAAGAATCAATGTAA56320.6665349060969246No Hit
TTGAGTGCCTATATCATGCAGGAACCCAGCACAGGAAGGCTCATCTTTGG55010.6510313420524115No Hit
TGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCCT55010.6510313420524115No Hit
TTGAGTGCCTATAGAAGATAAGTTTTGTATTTCCATCTAAATAGATACAT48270.5712649133043065No Hit
TTGAGTGCCTATAAACAGGACCCAAAGTGAGCCCTATATCATGAAGGAAC46660.552210914745783No Hit
TTGAGTGCCTATACCAAAGGGGGAAAAATACATGCATATTCTGCAGTCTT39650.46924909493506844No Hit
TTGAGTGCCTATAGCTGCAGTATTTTCTCTGCCCTCTTTCCAGTAGGGAC36270.4292475327438823No Hit
TTGAGTGCCTATACTTGGGGCAGAAGAGGTCCACAGACTAACCAAAAGAT36100.4272356198526096No Hit
TTGAGTCCCTGGAGAGTCTATCCAAGGTCAAGACCCACAGCCATAGATAG35540.42060814209312314No Hit
TTGAGTTCCTTCATGATATAGGGCTCACTTTGGGTCCTGTTTGTAGCTAA34740.4111403167224282No Hit
TTGAGTGCCTATAGGGGAGTGAGCTCATCCCAGAGGGAATGAGGAGAGAG34190.4046311867800754No Hit
TTGAGTGCCTATACCCCGATGACAGGGCTGTGAAGAAATGTGCCTAGATA33130.3920863181639046No Hit
TTGAGTCACATTCAGTTGGTGATTTACTTGGGGCAGAAGAGGTCCACAGA32620.3860505794900866No Hit
TTGAGTTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC32580.38557718822155185No Hit
TTGAGTGCCTATACCCTTGGTCATGGTTCATTACAGGGAAAAGATACAGA32580.38557718822155185No Hit
TCAAGGGATCCCTTACCTACTCTTAGCCTCCGTTTCCTTTGCTGTAACAG32170.3807249277190707No Hit
TTGAGTGCCTATATGTGGGTCTTACCTGCAACTGTTTGGAAATCCAAAAT28690.33953988735654766No Hit
TTGAGTGCCTATAACATTCATTAAGGCAAGCTCACGTGTGCAAAAATCTC28340.3353977137568686No Hit
TTGAGTGCCTATACGTTCATTAAGGCAAGCTCACGTGTGCAAAAATCTCT27820.3292436272659169No Hit
TTGAGTGCCTATAGGGGGAAAAATACATGCATATTCTGCAGTCTTTGATG27270.3227344973235641No Hit
TTGAGTGCCTATATCATGGAGGAACCCAGCACAGGAAGGCTCATCTTTGG26160.30959788962172485No Hit
TTGAGTCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGCTA25720.30439058566784266No Hit
TTGAGTCCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG25380.3003667598852973No Hit
TTGAGCCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGGT25180.29799980354262356No Hit
TTGAGTGCCTATATCGTGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG25150.2976447600912225No Hit
TTGAGTGCCTATATTCTGCAGTCTTTGATGAGTCACATTCAGTTGGTGAT24630.29149067360027076No Hit
TTGAGTGCCTATAACCCTTGGAAGAATCAATGTAAGCTGGTTAGCTACAA20700.24497998146673183No Hit
TTGAGTCTAAATTTTGGATTTCCAAACAGTTGCAGGTAAGACCCACATGT18120.21444624464624062No Hit
GCCGCCTTACACTGACTTGAGGTCACACAGCTGCTTCCAGGGCCTCAGAT15550.1840308556428831No Hit
TTGAGTGCCTATAGTAAAAACAGTGGCACTATTGCTAGGCAGTTCATGCC15490.18332076874008096No Hit
TTGAGTCCAAGCCAGCAGACTCCACATCCAAAGGCAAACCACCTAGTGGA15430.18261068183727885No Hit
TTGAGTCTTCCTGTGCTGGGTTCCTTCATGATATAGGGCTCACTTTGGGT14680.17373459555225235No Hit
TTGAGTGCCTATAGTGGACTAAGGATCAGCAGAGGCTGCAGACTGCGAGA14290.16911903068403855No Hit
TTGAGTACAAGCTTCCTTACCACTCCTGTTTTATTTAAAACTCCGCTCCT14100.1668704221584985No Hit
TTGAGTGCCTATATTGCTAGGCAGTTCATGCCATTTGAGAATTTGAACCC13900.16450346581582476No Hit
TTGAGTGCCTATATTCATTAAGGCAAGCTCACGTGTGCAAAAATCTCTGA13410.1587044227762741No Hit
TTGAGTGCCTATATTTTGCATAACGCATTTACAAATTTTGCTATTTAGTC12420.1469879888800391No Hit
TTGAGTGCCTATACTTTGCAATGAAAAGGACATGTGGGTCTTACCTGCAA12400.1467512932457717No Hit
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAACCCAGCATCC12140.14367425000029588No Hit
TTGAGTTCCAAACAGTTGCAGGTAAGACCCACATGTCCTTTTCATTGCAA11650.13787520696074523No Hit
TTGAGTGCCTATAAGATACTGATCAATTTGATAACCCTTGGAAGAATCAA11190.13243120737259562No Hit
TGAGTTGATAACCAAAGATGAGCCTTCCTGTGCTGGGTTCCTTCATGATA10400.12308172981903434No Hit
TGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGCT10300.12189825164769749No Hit
TTGAGTGCCTATAGAAGACAAGTTTTGTATTTCCATCTAAATAGATACAT9880.11692764332808263No Hit
TTGAGTTAAGATCTGAATGGTGGATCAGAAGAAGGCAGCTCAGAGTGGAT9380.1110102524713983No Hit
TTGAGTGCCAAAGGCTTTTGTTAGATTGTGAGGCAAGGTAGAGGGCAGGG9310.11018181775146248No Hit
TTAAGGCAAGCTCACGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATAC9040.10698642668885293No Hit
TTGAGTGCCTATAGAGGTCCACAGACTAACCAAAAGATACTGATCAATTT8790.10402773126051076No Hit
TTGAGTGCCTATAACCCCGATGACAGGGCTGTGAAGAAATGTGCCTAGAT8720.10319929654057496No Hit
TTGAGTACCATATGACCCTAAGAAGAACCCAAGGAATGCAGTATTAGGAC8720.10319929654057496No Hit
TTGAGTCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGCT8650.10237086182063916No Hit
TTGAGTGCCTATAGAAGCTAAGTTTTGTATTTCCATCTAAATAGATACAT8530.10095068801503491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGGT100.0072238077144.100561
TTGCGGT151.2608047E-4144.100561
CGAGATT100.0072276453144.074952
GAGTCAT100.0072327643144.040853
GAGTTCG100.0072327643144.040853
AGTCTAC151.2631858E-4144.032324
AGTACGA100.0072340444144.032324
AGTGTCC151.2631858E-4144.032324
AGTCGAA100.0072340444144.032324
AGTTTCG500.0144.03234
CCTTACG306.82121E-10143.998238
AGCCCGT100.007239166143.998239
TGGCGAT100.007239166143.998236
GTTAGAG100.007239166143.998235
TCGATAA151.2643776E-4143.998236
ATCCGTA100.007239166143.998236
GTTACTG202.2052045E-6143.998235
CTAAACG100.007239166143.998239
TCTAACG202.2052045E-6143.998236
TCCGTAA100.007239166143.998237
Produced by FastQC (version 0.11.2)