FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_ny238_plasmid_mix_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_ny238_plasmid_mix_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673657
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC636619.450061381385483No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT569868.459201047417306No Hit
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG336434.994084526695336No Hit
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT322184.7825525452864No Hit
TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC165302.4537709843436644No Hit
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG162902.418144545369528No Hit
TTGAGTGCTTTTGAGATCTGCTGCTTGTCCTATTCCCTCAGAATTTTGAT126651.8800368733643384No Hit
TCAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTT126311.874989794509669No Hit
TTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAA116121.7237258723653135No Hit
TTGAGCATATAGGCTGATTTGGTCATTGTCCGTACCCGGGTGGTGAACTC106531.5813685599644922No Hit
TTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA101851.5118970039649258No Hit
TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA98181.4574182410336418No Hit
TTGAGTAGCTAAGACCCCGTAATTGATTACTATTAATAACTAGTCAATAA86611.2856691164791578No Hit
TTGAGTAGCTAAGGACCCCGTAATTGATTACTATTAATAACTAGTCAATA82521.2249557267273998No Hit
TTGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTG76451.1348505248219791No Hit
TTGAGTAGCTAAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAG66530.9875945770622141No Hit
TTGAGCATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATA49690.737615730260355No Hit
TTGAGTGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA48770.7239589286536026No Hit
TTGAGTAGCTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAC45260.6718552616539277No Hit
TTGAGTAGCTAAGCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG45190.6708161571838488No Hit
TCAAGCATCAGGAAGAATCACAGTCTCTACCAAAAGAAGCCAACAAACTG29590.4392443038519603No Hit
TTGAGTAGCTAAGAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA29520.43820519938188124No Hit
TTGAGTATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATA26760.3972347945616241No Hit
TTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCC25640.3806091230403603No Hit
CAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGT17190.25517436915225405No Hit
TTGAGTAGCTAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCA17100.2538383776907239No Hit
TTGAGTAGCTAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCA15320.22741543545157253No Hit
TTGAGTAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCT14750.21895415619521508No Hit
TTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA14490.2150946253063503No Hit
TTGAGTAGCTAAGAATGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA14470.21479773831489915No Hit
TTGAGTAGCTAAGCACAGGGAATCTAATTGCTCCTCGGGGTTACTTCAAA14370.21331330335764342No Hit
TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC14240.21138353791321104No Hit
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT14110.20945377246877864No Hit
TTGAGTAGCTAAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTT14070.20885999848587636No Hit
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG13620.2021800411782257No Hit
TTGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG13420.19921117126371432No Hit
TTGAGGATGAACAGATGTACCAAAAGTGCTGCAACTTGTTCGAGAAATTT12560.18644503063131534No Hit
TCGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCG11870.17620242942625106No Hit
TTGAGTAGCTAATAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA11780.1748664379647209No Hit
TTGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCT11500.17071002008440497No Hit
TCAAGGATGTGATGGAATCAATGGATAAAGAGGAAATGGAGATAACAACA10750.15957675790498727No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAGCGGTTCCTCTTGGG10700.15883454042635942No Hit
TTGAGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT10670.1583892099391827No Hit
TTGAGGAAATCTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGA10660.15824076644345714No Hit
TTGAGTAGCTAAGAGAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA10250.15215458311870877No Hit
TTGAGTAGCTAAGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTG10050.14918571320419738No Hit
TTGAGTGACTCCAGTCCAATTGAAGCTTTCATTGTTAAACTCCAGTGTGC9720.1442870778452536No Hit
TTGAGTAGCTAAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT9620.1428026428879979No Hit
TTGAGTAGCTAAGAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAA9570.14206042540937006No Hit
TTGAGTAGCTAAGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAG9470.14057599045211436No Hit
TTGAGTAGCTAAGCCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCC9430.13998221646921208No Hit
TTGAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTAC9400.13953688598203537No Hit
TTGAGTAGCTAAGATAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA8060.11964545755480906No Hit
TTGAGTAGCTAAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATT7760.11519215268304195No Hit
GCCGCCTTACACTGACTTGAGTGGCGAAACCCGACAGGACTATAAAGATA7740.11489526569159084No Hit
TTGAGTAGCTAAGAAAGGGAATTTATTTTCATTGCAATAGTGTGTTGGAA7710.11444993520441413No Hit
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCA7600.11281705675143285No Hit
CAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA7070.10494955147797766No Hit
TTGAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTA7070.10494955147797766No Hit
TTGAGTAGCTAAGAATGGTTACAAATAAAGCAATAGCATCACAAATTTCA6900.10242601205064299No Hit
TTGAGTAGCTAAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTT6820.10123846408483841No Hit
TTGAGTAGCTAAGTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATA6810.10109002058911284No Hit
TTGAGTAGCTAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAATTTT6810.10109002058911284No Hit
TTGAGTAGCTAAGAAGGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA6780.10064469010193616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTAG100.007236021144.0172
GTCTAAC100.007237627144.006325
TCTAGGC100.007237627144.006326
GATATTA100.007237627144.006326
TCAAGCA5800.0141.554961
TCAAGAC38150.0140.640371
TCAAGGA24500.0139.922991
TATAGGC13100.0139.609188
GAGGATG1450.0139.050893
TCAAGTC53400.0138.913421
GAGAGTT12900.0136.760333
GTAGTAA2900.0136.557715
CTTACAC20200.0136.520846
GCGTCGA14300.0136.453525
CGTCGAT14100.0136.34646
GAGCATA12850.0136.17173
GCATATA12800.0136.130975
TTGAGAG13650.0136.124191
GCCTTAC20500.0135.92794
GAGTCCA70250.0135.919253
Produced by FastQC (version 0.11.2)