FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_ny238_plasmid_mix_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_ny238_plasmid_mix_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829872
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT43410.23722970787027728No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG42210.23067187213094684No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG41280.22558954943296577No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG40940.22373149597348885No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG40300.2202339835791793No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA35970.19657112628642878No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC34980.1911609118014812No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC34530.18870172339923227No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT33800.18471237332447296No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT31310.17110486416536239No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC29960.16372729895861568No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT29140.15924611120340657No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT28760.15716946321928527No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT28270.15449168029239205No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG26080.14252363006811405No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT25690.14039233345283167No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT25320.13837033409987148No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT24570.13427168676278997No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG23980.13104741752428584No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA23090.12618368935094915No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA21820.11924331319349113No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT20260.1107181267323616No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT20240.11060882947003944No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA20190.110335586314234No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG19620.10722061433805206No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT19400.10601834445250816No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC19290.1054172095097362No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG18780.10263012932052078No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT18490.10104531901684927No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT18350.10028023818059405No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA18330.10017094091827189No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC702.4469773E-761.7257423
TCGGACA806.158407E-754.0070724
TACTGAT13850.049.387357
CTTCTAA15650.046.49161
CATATAC15100.045.3064233
TATACTG24050.043.4116865
TACTGTG12600.042.8580677
TTCTAAT17250.042.587642
TAGATGG17450.042.086878
GTACTGT14600.036.986096
GTATATA20800.034.6341171
TATATAT21100.034.4755632
CCTTATA10650.033.8137132
GTATATG14800.033.585731
ATGATAG12450.033.5438085
TTATACT10900.033.031854
ATAGCGA15700.033.018926
TAGCGAT15750.032.9149977
TGATAGG12700.032.3147776
TGAGTAA22400.032.143558
Produced by FastQC (version 0.11.2)