FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_lp_negative_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_lp_negative_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182
Sequences flagged as poor quality0
Sequence length150
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTT761.8173122907699666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG651.5542802486848397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTT400.9564801530368245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTT330.7890961262553802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGTTTTTTTTTTTTTTTTTT210.5021520803443328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTGTTTTTTTTTTTTT130.31085604973696795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTTTTTTTTTTTTTTTTTTT120.2869440459110474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTTTTTTTTTTTTTTTTTT100.23912003825920614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTGT100.23912003825920614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGTTTTTTTTTTTTTTTTTTT100.23912003825920614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTTTTTTTTTTTTTTTTTT90.2152080344332855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTTTTTTTTTTTTTTTTTT90.2152080344332855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGAGGGTTTTTTTTTTTTTTTTTTT80.19129603060736491No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTTTTTTTTTTTTTT80.19129603060736491No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGTTTTTTTTTTTTTTTTTTT80.19129603060736491No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGGTTTTATTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTGTTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGTTTTTTTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGTTTTATTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTGTTTTTTTTTTTTTT70.1673840267814443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTGTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTTTTTTTTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGTTTTTTTTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGGTTTTTTTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTATTTTTTTTTTTTT60.1434720229555237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAGTGTTTTTTTTTTTTTTTTTTT60.1434720229555237No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT50.11956001912960307Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGTGAGGGGTTTTATTTTTTTTTTTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTTTTTTTTTTTTTTTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGGTTTTTTTTTTTTTTTTGT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGTTTTTTTTTTTTTTTTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTGTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGAGGGGTTTTTTTTTTTTTTTTTT50.11956001912960307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGT50.11956001912960307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTGG151.1762792E-4144.000026
GAGTGGG100.006983203144.07
GGGAGTG203.6980276E-4108.05
GGGCGGG804.794765E-754.09
GGGGGCG906.533055E-540.0000047
GGGGCGG953.5243174E-537.8947378
GGGGAGT602.4003511E-436.0000044
GGGGGGC1102.1302613E-432.7272726
AGGGTTT200.0061445228.80000125-29
GAGTTAA200.0061445228.80000165-69
GGGAGAG200.0061445228.80000120-24
TGGGGGA200.0061445228.80000120-24
CGCGGGG200.0061445228.80000110-14
AATATTT200.0061445228.80000135-39
GAATAAA304.4001787E-528.80000165-69
GGGAAGA200.0061445228.80000120-24
GGGTGAG200.0061445228.80000120-24
GGAGTTA200.0061445228.80000165-69
AGTTAAA200.0061445228.80000165-69
AGGGGTT552.0008883E-1028.825-29
Produced by FastQC (version 0.11.2)