FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_lp_negative_control_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_lp_negative_control_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216.666666666666664No Hit
GTATTGGGATAGAAAAGGTACAAGATGGTGGTAAAACGACTTGGCACTCA18.333333333333332No Hit
GGATTCTGTTTGCCATGTCACTCTTGGTCAAAGCCAAGGGAAAATTAGAA18.333333333333332No Hit
GTTCCATCTGGTGCCTCCACCGGTGTCCACGAAGCTTTGGAAATGAGAGA18.333333333333332No Hit
CATTGGCCTGACCAAGGCCACATCCAGATCTGTACAGTTGAGACAGTCTG18.333333333333332No Hit
ACATATGCCCATATGCTGGCTGCCATGAACAAAGGTTGGCTATAAAGAGG18.333333333333332No Hit
CCAAAAGAATGGGGATGGGACTGTATGTAAAGTATGATGGAGACCCATGG18.333333333333332No Hit
CACGAGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG18.333333333333332No Hit
GGTAAAGTGACTGCCCAGTCAATAAGTAATGGGTGCCTGCTTGGGATTTC18.333333333333332No Hit
CTCTTCCCTAGTAAACATGTAGCTGGATTCGTTTATGTCAATATTTATCC18.333333333333332No Hit
AGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)