FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_cal-09_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_cal-09_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807800
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGAC711198.804035652389205No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGT516576.394775934637287No Hit
TTGAGTTCATCTGGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGAC319393.95382520425848No Hit
TTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGG237232.936741767764298No Hit
TTGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCT231502.8658083684080218No Hit
TCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATA182782.2626887843525627No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGG150331.8609804407031443No Hit
TTGAGTTAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGC117481.454320376330775No Hit
TTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTG95151.1778905669720228No Hit
TTGAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTA93911.1625402327308738No Hit
TTGAGTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA86421.0698192621936122No Hit
TTGAGTTAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA79300.9816786333250804No Hit
TTGAGTTCATCTGTCCCTTTTCTATTCTGAAGATCTTGTATGAGGCCTGT57880.7165139886110423No Hit
TTGAGGATGAACAGATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTT50420.624164397128002No Hit
TTGAGTCAAGAAGAAGGTTCTGCATTCCAAGGGGGAGCATGATATGAATG47100.5830651151275068No Hit
TCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGC44890.5557068581332013No Hit
TCAAGGCACCAAACGATCATATGAACAAATGGAGACTGGTGGGGAGCGCC43550.5391185937113148No Hit
TTGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAA42850.53045308244615No Hit
TTGAGTTCATCTGGGGGGTGCGAAAGCAGGGTGACAAAAACATAATGGAC41280.5110175786085666No Hit
TTGAGTTCATCTGGGGGGGGCGAAAGCAGGGTGACAAAAACATAATGGAC40710.503961376578361No Hit
TGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATC39160.484773458776925No Hit
TTGAGTTCATCTGAAGATCTTGTATGAGGCCTGTCCATTACTTGGTCCAT35310.4371131468185194No Hit
TTGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTC32990.4083931666254023No Hit
TTGAGTTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATA31820.39390938351077004No Hit
TTGAGTTCATCTGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTT30760.38078732359494927No Hit
TTGAGTTCATCTGGGTGACAAAAACATAATGGACTCCAACACCATGTCAA29550.3658083684080218No Hit
TTGAGTTCATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA29080.3599900965585541No Hit
TCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAG27450.33981183461252784No Hit
TCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCAT26280.3253280514978955No Hit
TCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCAGTCTCTGCGCGAT25320.31344392176281255No Hit
CGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTC24860.30774944293141865No Hit
TTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTC24330.3011884129735083No Hit
TTGAGTTCATCTGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT24250.30019806882891803No Hit
TTGAGTGCATCTGTCGGAAGAATGATTGGTGGAATCGGGAGATTCTACAT24010.2972270363951473No Hit
TTGAGTTCATCTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACC22230.27519187917801435No Hit
TGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT17870.22121812329784601No Hit
TTGAGTTCATCTGGGGGGTGCAAAAGCAGGGTGACAAAAACATAATGGAC17800.22035157217132956No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGT17680.2188660559544442No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGATACAGATGCAATTGT17240.21341916315919782No Hit
TTGAGTTCATCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTAT17020.21069571676157464No Hit
TTGAGTTCATCTGGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGA16830.20834364941817282No Hit
TTGAGTCAATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACT16650.20611537509284475No Hit
TTGAGTTCATCTGATTTGACTATCTTTCCCTTTTCTATTCTGAAGATCTT16230.20091606833374598No Hit
TTGAGTTCATCTGGGGGGGGCAAAAGCAGGGTGACAAAAACATAATGGAC15400.19064124783362218No Hit
TTGAGTTCATCTGAGGGGGGAGCAATAGTTGGAGAAATTTCACCATTACC15070.18655607823718742No Hit
TTGAGTTGTATGGAGAGGGAACTTCACCAATAGGACAGCTCATTAGGGTT14350.17764298093587522No Hit
TTGAGTTCATCTGGGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACC14050.1739291903936618No Hit
TTGAGTTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAG13850.17145333003218619No Hit
TTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGGAAT12660.15672196088140627No Hit
TTGAGTTCATCTGGGATCCATTCCGGTGCGAACAAGCGCTCTTGTTCTCT12580.15573161673681604No Hit
TTGAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCT12540.1552364446645209No Hit
TTGAGTTTATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA12110.14991334488734834No Hit
TTGAGTTCATCTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGA11900.14731369150779897No Hit
TTGAGTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCA11800.14607576132706115No Hit
TTGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGG11520.1426095568209953No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTC11300.13988611042337212No Hit
TCAAGGGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACA11240.13914335231492944No Hit
TTGAGTTCATCTGGGGGGCGCGAAAGCAGGGTGACAAAAACATAATGGAC11160.13815300817033918No Hit
TTGAGTTCATCTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATT11070.13703887100767517No Hit
TTGAGTTCATCTGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACT11040.13666749195345382No Hit
TTGAGTTCATCTGGAGGGGTGAAAATGGACGAAGGACAAGGGTTGCTTAT10920.13518197573656845No Hit
TTGAGTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACTCTCCTTATTTC10870.13456301064619955No Hit
TTGAGTTCATCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGG10610.13134439217628127No Hit
TTGAGTTCATCTGGGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGA10420.12899232483287942No Hit
TTGAGTTCATCTGGTTGAAAGACACCCACGGTCGATTCGAGCCATGCCAG10130.1254023273087398No Hit
CGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAA10030.124164397128002No Hit
TTGAGTTCATCTGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTC9850.12193612280267394No Hit
TTGAGTTCATCTGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGATTC9420.11661302302550136No Hit
TTGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCAC9410.11648923000742759No Hit
TTGAGTGCATCCGCAGGCCAGATCAGTGTGCAGCCTACATTCTCAGTGCA9390.11624164397128No Hit
TTGAGTTCATCTGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTC9210.11401336964595196No Hit
TTGAGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCA9080.11240406041099282No Hit
TTGAGTTCATCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAG9080.11240406041099282No Hit
TTGAGTGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTC8790.10881406288685318No Hit
TTGAGTTATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT8680.10745233968804159No Hit
TTGAGTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT8320.10299579103738549No Hit
TTGAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAA8220.10175786085664769No Hit
TTGAGTTCATCTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGC8090.10014855162168854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGCT100.0072311964144.05092
GAGTATA351.2732926E-11144.024143
GTTAGTC351.2732926E-11144.015235
ACCTGAC100.007237892144.006329
ATCCGGT151.2640744E-4144.006329
TCGAGGG62300.0142.565611
GCCGCCT20950.0140.286511
CGCCTTA20950.0140.243093
CCGCCTT21050.0139.26062
TTGAGTA51600.0138.624281
TTGAGTT453600.0137.732391
CGAGGGT65100.0136.306232
GAGTTCA359950.0136.121723
GAGGGTC65550.0135.674933
AGGGTCA65450.0135.543754
GCCTTAC21700.0135.387594
CATCTGG232550.0134.925968
TTCATCT342750.0134.670656
TGAGTTC375800.0134.410452
GGATGAA6500.0134.044955
Produced by FastQC (version 0.11.2)