FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_463_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_463_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820088
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA801299.77078069670572No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC750279.148652339748905No Hit
TTGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC557386.796587683273014No Hit
TTGAGTTAACCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG317673.873608685897123No Hit
TTGAGTTAACCAGGGGAGCATGCAAAGGCCATAGGAAATTGCCCAATATG208122.5377764337485735No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC175272.137209665304211No Hit
TTGAGTTAACCAGGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATG151311.8450458975134376No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC149901.8278526206943646No Hit
TTGAGTTAACCAGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATGG144701.7644447912906907No Hit
TTGAGTTAACCAGGGGACCATGCAAAGGCCATAGGAAATTGCCCAATATG135491.6521397703661072No Hit
TTGAGTTAACCAGATTGCCTCCACGAAAAATACGGTGGATTAAACAAAAG120481.4691106320297334No Hit
TTGAGTTAACCAGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGG112511.3719259396552563No Hit
TTGAGTTAACCAGGGGATCATGCAAAGGCCATAGGAAATTGCCCAATATG77760.9481909258518597No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG72120.8794178185755674No Hit
TTGAGTTAACCAGGGGAATATGCAAAGGCCATAGGAAATTGCCCAATATG68200.831618070255875No Hit
TTGAGTTAACCAGGAGACCAATATAACATAATAAGAAAATTCAATACATC64990.7924759294124534No Hit
TTGAGTTCATCCATGGCACCGCTTAGTCTTTGAAGATTCTTTACTTCCAG61040.744310366692355No Hit
TTGAGTTAACCAGGGGTACATGCAAAGGCCATAGGAAATTGCCCAATATG52120.6355415516383608No Hit
TTGAGTTAACCAGTTTTGAAGCAAACAACAGTAGACCAATATAACATAAT47810.5829862161133927No Hit
TTGAGTACCAGCAGCTATTCTGTCGAGACAGGTCTGGTTGCACTTGTGTT46290.564451619826165No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT41150.5017754192233028No Hit
TTGAGTTAACCAGAAGACGGAGGACTACCACAAAGTGGTAGAATTGTTGT35980.438733404220035No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA35550.43349006448088495No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA32640.3980060676415214No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTCTT31330.38203217215713436No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCCAATGCAGC30900.3767888324179844No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG30620.3733745646808635No Hit
TTGAGTTAACCAGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC27300.3328911043692872No Hit
TTGAGTTAACCAGGGGCACATGCAAAGGCCATAGGAAATTGCCCAATATG27300.3328911043692872No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGGGTCTTGTTGTCAGAGTGGAAC26860.32752582649666867No Hit
TGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAAA26670.3252090019607652No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA21820.2660690072284926No Hit
TTGAGTTAACCAGTTGACCAATATAACATAATAAGAAAATTCAATACATC21420.2611914818897484No Hit
TTGAGTTAACCAGAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA20920.2550945752163183No Hit
TTGAGTTAACCAGGTGAACATGCAAAGGCCATAGGAAATTGCCCAATATG20670.2520461218796032No Hit
TGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATCA20230.2466808440069846No Hit
TTGAGTTAACCAGGGAAACATGCAAAGGCCATAGGAAATTGCCCAATATG19790.24131556613436606No Hit
TTGAGTTCCAATACTGAAAGTTTTGGGGCTATTTACTCTGTCTCCCTTGA18890.23034113412219176No Hit
TTGAGTTTGGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCC17780.21680600130717678No Hit
TTAAGGAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT17450.21278204290271288No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG15630.19058930261142706No Hit
TTGAGTTAACCAGGAGAACATGCAAAGGCCATAGGAAATTGCCCAATATG15480.188760230609398No Hit
TTGAGTTAACCAGAGGCCATAGGAAATTGCCCAATATGGGTGAAAACACC15390.1876627874081806No Hit
TTGAGTTAACCAGTAGCCCAATATAACATAATAAGAAAATTCAATACATC15190.18522402473880853No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA15150.1847362722049341No Hit
TTGAGTTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATATTCAGT14210.17327408765888538No Hit
TTGAGTTTACCAGCAGCTATTCTGTCGAGACAGGTCTGGTTGCACTTGTG13960.1702256343221703No Hit
TTGAGTTAACCAGCAGCTATTCTGTCGAGACAGGTCTGGTTGCACTTGTG13670.1666894284515808No Hit
TTGAGTTAACCAGGGGGACAACCAGACCAATCATTAGACCAGCAACCCTT13460.16412872764874012No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT13010.15864151164265297No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA12610.15376398630390886No Hit
TTGAGTTAACCAGTGGACCAATATAACATAATAAGAAAATTCAATACATC12380.15095940923413098No Hit
TTGAGTTAACCAGGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAA12300.14998390416638216No Hit
TTGAGTCAACCAGTTCTCTTTCAAGCATGTATGCCAGTACGATTGGAGTT12250.14937421349903912No Hit
TTGAGTTAACCAGAAATAGTATTAAAGTATAATCTAATGGACCCTGAATA12050.14693545082966705No Hit
TTGAGTTAACCAGTGGGACAACCAGACCAATCATTAGACCAGCAACCCTT11120.13559520441708695No Hit
TTGAGTTAACCAGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCC10900.13291256548077768No Hit
TTGAGTTAACCAGGGGAGATTCCAAAGTAGAAGGAAAGAGAATGAAAATC10050.12254782413594638No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA10020.12218200973554058No Hit
TTGAGTTAACCAGGAGGGAATAAATTAACTGAGTCCAGGTCTCAATCAAT9880.12047487586698014No Hit
TTGAGTTAACCAGGGGGATACTACAATGGAACAAGAGGAGACAGAAACAA9640.11754836066373364No Hit
TTGAGTTAACCAGAGTGGAATACATCAAAAGAGCATTATCATTAAACACA9610.11718254626332783No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC9540.11632897932904761No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT9480.115597350528236No Hit
TTGAGTTAACCAGGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT9100.11096370145642906No Hit
TTGAGTTAACCAGCAACCCTTGCCCCACCAAGCAACAAACGAACCCGTAA8730.10645199051809073No Hit
TTGAGTAAACCAGCAATAGCTCCGAAGAAACCCCTTTCCTTTAATAGTTT8580.1046229185160617No Hit
TTGAGTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATCA8420.10267190838056403No Hit
TTGAGTTAACCAGAAGACCAATATAACATAATAAGAAAATTCAATACATC8230.10035508384466058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACT100.007229563144.061921
CTATCTG151.2636733E-4144.017999
ATTCGAG1450.0144.009226
TGACCCG100.0072374768144.009227
TATCCCG151.2639804E-4144.00927
GCCGCCT19600.0142.59191
CGCCTTA19850.0140.778853
CTTACAC20000.0140.769016
TTGAGTT535050.0140.19821
CGTCCAA3600.0140.008967
TTACACT20200.0139.73177
CCGTCCA3600.0138.008826
CCGCCTT20200.0137.991492
AGTTAAC398550.0137.213494
GTTAACC392050.0137.103525
GAGTTAA409900.0136.840383
TGAGTTA518600.0136.775472
TTAACCA388150.0136.755876
CCTTACA20450.0136.615085
TTAAGGT91600.0136.27691
Produced by FastQC (version 0.11.2)