FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_463_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_463_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962256
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC104200.5310214365505825No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT76400.3893477711368955No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA29640.15105062744106784No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT29380.14972562193719882No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA28610.14580156717574058No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27910.142234244665324No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC25310.12898418962663383No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25140.12811783987410408No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT24900.1268947578705327No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG24170.1231745501096697No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT22260.1134408558312473No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC22250.11338989408109848No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA21470.10941487756949145No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC20700.1054908228080332No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20340.10365619980267611No Hit
CACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG20100.10243311779910472No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20100.10243311779910472No Hit
CAACAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA20050.10217830904836066No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT19660.10019080079255714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA36650.0126.365444
TAACAAG42500.0108.943845
AGCACGC4950.098.967971
TAGTAAC47200.098.896222
CGCACTT5700.092.220745
GCACGCA5550.090.8556062
CACGCAC5550.090.8486563
GAAACAC18400.090.0329064
ACAAGAG51400.089.935347
AAACACG18700.088.180895
CGGTGCA2050.087.848192
TAGAAAC21900.086.179482
AGTAACA55400.084.7717743
ACGCACT6800.078.38164
ACGTGCA9450.074.70352
CAAGAGC54600.073.9824458
AACACGT5200.069.236676
CTCGCTC1050.068.63
CGGTGCG3500.067.9192052
GGTGCGT3650.065.123023
Produced by FastQC (version 0.11.2)