FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_460_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_460_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471860
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC23153615.730843966138083No Hit
TTGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA1364829.272756919815743No Hit
TTGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA976566.63487016428193No Hit
TTGAGTTATCCGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTG619414.208348620113326No Hit
TTGAGTTATCCGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA457733.1098745804628156No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG350642.3822917940565No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA326022.2150204503145683No Hit
TTGAGTTATCCGCATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA159321.0824399059693177No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG150171.0202736673324908No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA129260.8782085252673488No Hit
TTGAGTTATCCGCAATGGCCATAGGAAATTGCCCAATATGGGTGAAAACA121410.8248746484040602No Hit
TTGAGTATCTGTGGGCATCAGGAGGAGGAGTTCATGAGAGGACAGGCTGG101890.6922533393121629No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGGGTCTTGTTGTCAGAGTGGAAC93310.6339597516068105No Hit
TTGAGTTATCCGCCTCCACGAAAAATACGGTGGATTAAACAAAAGCAAGC91350.6206432677020912No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC84180.5719293954588072No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG80730.548489666136725No Hit
TTGAGTTATCCGCAGAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA73630.5002513826043238No Hit
TTGAGTTATCCGCTGGCCAATGGAACCAAATATAGACCTCCTGCAAAACT66390.45106192165015696No Hit
TTGAGTTATCCGCGGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA62460.4243610125963067No Hit
TTGAGTTATCCGCATAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA59900.4069680540268776No Hit
TTGAGTCCTCATCTCGTCCAGCTGGCAGGGAAATCCAACTTATTACTCCT58320.396233337409808No Hit
TTGAGTTATCCGCAACGGCCATAGGAAATTGCCCAATATGGGTGAAAACA52740.35832212302800537No Hit
TTGAGTTATCCGCAAGGGCCATAGGAAATTGCCCAATATGGGTGAAAACA50010.33977416330357507No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT49520.33644504232739525No Hit
TTGAGTTATCCGCAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAG45740.31076325193972254No Hit
TTGAGTTATCCGCACAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA40940.2781514546220428No Hit
TGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT40260.2735314500020382No Hit
TTGAGTTATCCGCTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC40100.27244439009144894No Hit
TTGAGTTGTCCACCTGGACCCAATGTCATCACGAAAGTCCTTCTAAGAAG40040.27203674262497796No Hit
TTGAGTTATCCGCGAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA34350.23337817455464516No Hit
TTGAGTTATCCGCGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT33720.2290978761566997No Hit
TTGAGTTATCCGCCAGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAG33180.22542904895846072No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA32900.2235266941149294No Hit
TCAAGCTCACTCTCCGCCACCCCTAAAGACCTCTTCCTCCCTCCTCTGAA31110.21136521136521136No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT31080.21116138763197587No Hit
TTGAGTTATCCGCTGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA30870.2097346214993274No Hit
TGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAAT29230.19859225741578682No Hit
TTGAGTTATCCGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGC28930.19655402008343184No Hit
TTGAGTTATCCGCCATGGGAAATTGCCCAATATGGGTGAAAACACCCTTG28480.19349666408489938No Hit
TTGAGCATCCCAAAAAACTGTTGCCACAACCTTTGCTCTTGACTGGTCCA26140.17759841289253053No Hit
TTGAGTTGATAACCTGATATGTTCGTATCCTCGGAGAAGGTTAGGCAGCT25860.17569605804899924No Hit
TTGAGTTATCCGCCACGAAAAATACGGTGGATTAAACAAAAGCAAGCCTT24320.165233106409577No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC24310.16516516516516516No Hit
TTGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCGAACTAATTTTTT24120.16387428152134034No Hit
TTGAGTTATCCGCAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACAC22630.15375103610397728No Hit
TTGAGTTATCCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCT21670.14722867664044134No Hit
TTGAGTTATCCGCCGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA21170.1438316144198497No Hit
TTGAGTTATCCGCCAATGGAACCAAATATAGACCTCCTGCAAAACTATTA21080.1432201432201432No Hit
TTGAGTTATCCGCAAAGGTCATAGGAAATTGCCCAATATGGGTGAAAACA20740.1409101409101409No Hit
GCCGCCTTACACTGACTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACT19730.13404807522454582No Hit
TTGAGTTATCCGCACACCCCAGATGCCCTGGCACTATGGACGGCACCAGG19340.13139836669248434No Hit
TCAAGATTCTCAGGTGGTCAGCTGGTTCTTCTACTGGTGTTTTCTATCCC18890.12834101069395187No Hit
TTGAGTTATACGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA18330.12453630100688923No Hit
TTGAGTTACCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA18300.12433247727365374No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA18130.12317747611865258No Hit
TTGAGTTATCCGCAGGGGGACATGCAAAGGCCATAGGAAATTGCCCAATA17650.11991629638688463No Hit
TTGAGTTAGCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA17610.1196445314092373No Hit
TTGAGTTGGAAAAGGGCCTGCCTGCTGAAAGGCTCCAGTCCTGTGTGTTG17520.11903306020953079No Hit
TTGAGTTATACGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA17500.11889717772070713No Hit
TTGAGTTATCCGCGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACT16740.11373364314540785No Hit
TTGAGTAAAACACACTGAGGAATGCACATGCGGATTTGCCAGCAATAAAA16690.11339393692334869No Hit
TGAGTTATCCGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGA16450.1117633470574647No Hit
TTGAGTTATCCGCAAATGCCATAGGAAATTGCCCAATATGGGTGAAAACA16100.10938540350305057No Hit
TTGAGTTCATCCATGGCACCGCTTAGTCTTTGAAGATTCTTTACTTCCAG15740.10693951870422459No Hit
TTGAGTTATCCGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAA15610.10605628252687077No Hit
TTGAGTAATGGGCCATCTGGTGCTCTGGTTAGTGGCAGCAAGGCTGTATG15460.10503716386069328No Hit
TTGAGTTATCCGCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA15340.10422186892775129No Hit
TTGAGTTAGCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA15300.10395010395010396No Hit
TTGAGTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAATAGTTTTGAAA15300.10395010395010396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGG550.0144.025734
TACGCTA202.2036602E-6144.025739
TACGCGA202.2036602E-6144.025739
TTCGCGC253.8648068E-8144.025739
TTCGCGG151.2636055E-4144.025719
GTATCGC202.204777E-6144.011055
GTTATCG3000.0144.011055
AATCCGA100.007238517144.006157
TTTACGC100.007238517144.006157
ATTCGCG400.0144.006158
ATGCGCT306.82121E-10144.006158
TTACGCT202.20515E-6144.006156
TTACGCG100.007238517144.006156
TTGAGTT865800.0142.001571
TTGAGTA83000.0141.46131
ATACGCA7400.0139.141078
TATACGC13250.0138.571957
ATACGCG1300.0138.467458
TTATACG13400.0138.095446
GTTACGC1200.0138.010595
Produced by FastQC (version 0.11.2)