FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_460_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_460_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4715998
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC105270.2232189241810535No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC64250.13623839535131269No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA58750.12457596462085013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG57700.088.101895
AACACGT21100.084.2845156
AACACGA41200.081.262286
ACACGAG42300.079.148247
ACACGTG23150.076.820077
ACGAGCC19150.075.575959
GTAACAA23600.073.837694
CACGAGC46500.071.8445368
ACGTGCC9950.069.4711469
TAGAAAC99050.067.908952
GAAACAC78500.065.494274
CGCACTA1350.064.003515
ACGAGCT7550.061.9901549
GAAGCGG13050.059.6109621
CACGTGC30950.056.994658
CGGAGCG3200.051.752845
TAGTAAC35400.051.673172
GTAGAAA134550.051.3925861
AAGCGGA13900.047.6658822
AGTAACA40850.045.127483
Produced by FastQC (version 0.11.2)