FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_428_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_428_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566745
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC403777.12436810205648No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG327095.771378662361379No Hit
TTGAGTACTAACGGGGGGAAAATCATGGGAAAAATACTTAAAATGTCTAA137772.430899258043741No Hit
TTGAGTACTAACGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTAA119552.109414286848583No Hit
TCAAGGATTGGAGGTGGCAGCCCCACCTGGCAGACACCATCGTATATGCC107651.8994433122480128No Hit
TTGAGTGGTTCTCTATGTGTACATTGTCTTTGGAATGATTGAGAGGTGGA83301.4697968222039894No Hit
TTGAGTACTAACGTCACTAATTGTCAGGGAAATGCAAATATAAAACACAG79461.4020414825009484No Hit
TTGAGAAATCCCACAAGACCCTTGGCCTTACCCACCAGCAGACCAGCAGC72781.2841754228092No Hit
TTGAGTAACACAAACCAACTAACTTACAGATAGGCATTACCTTTTGGATT70841.2499448605633927No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG64771.1428420188973876No Hit
TTGAGTGGGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGCTCAG61381.0830267580657966No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA53600.9457516166882814No Hit
TTGAGTACTAACGCAGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT39570.6981976020961808No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA33930.5986819469073393No Hit
TCAAGAACCCCCATCCGTCTCCCTCCCTGCCTTACTGCTTCCACTCCTGC26770.47234646975271066No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT25990.45858366637553044No Hit
TTGAGTACTAACGCTGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT24620.43441053736689345No Hit
TTGAGTACTAACGGTTTGGATATAGTTAGTTAAGAAGGTGAGAGCTCAGT23970.42294153455257655No Hit
TTGAGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGTAA22340.39418080441821274No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT22220.3920634500524927No Hit
TTGAGTACTAACGTGCCCTAAAAACAAAACAAACAAAAATCTTGAACTAA21510.3795357700553159No Hit
TTGAGTACTAACGAGGCCAGGGATGTGGTATCCAGTTTACCTGTGAACAG20890.368596105832429No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT19720.3479519007666587No Hit
TTAAGAAGACAGAAGGGCAAAGCCACAGGTGGTAAAGTCATGATTGTTGA19320.3408940528809253No Hit
TTGAGTTCTAACGCAGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT19320.3408940528809253No Hit
TCAAGGACGTGAAGCAGTTCTGACAACCTGGGACCTCTTAAAGAGCCATT19250.33965892950092197No Hit
TTGAGTACTAACGCGGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT19000.33524777457233856No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT18820.33207174302375847No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT18370.3241316641523084No Hit
TTGAGTTAGGGAGTTACTGATCTCTCTGGTAACTGAACAGGTTTAGAAGT17530.30931018359226814No Hit
TCAAGTGTGGCCCTAGCACCAAGGAAGCTTCATTAGTGATTTACCTACTA16820.2967825035950913No Hit
TTGAGTACTAACGGCCAGGGATGTGGTATCCAGTTTACCTGTGAACAGCA16420.28972465570935785No Hit
TTGAGAGGGTGACATTTGAGCTGGTTTTGGGGTAACAGTTCACTAAAGAG16330.28813663993506783No Hit
TTGAGTACTAACGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGC16220.2861957317664911No Hit
TTGAGTACTAACGTTAAAGTTCAAACTCTTTGTGGAAAGAGGATTCATAG15660.2763147447264643No Hit
TTGAGTACTAACGCCGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT15560.2745502827550309No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT14810.2613168179692807No Hit
TTGAGTTCAAATGTGGCAGTGTTCCAGAAGTAGTACTGTGCTGCATATCA14560.25690566304069734No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT13510.23837881234064703No Hit
TCAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGA12770.22532179375204017No Hit
TTGAGTACTAACGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATG12550.22143997741488675No Hit
TTGAGTACTAACGGAAACAGCTGAATACAGCAAGAAACACACGCTTCTTA11920.2103238669948566No Hit
TTGAGTGAAGAAGGCACAGGTCTTAAAATAGGTTTTCTTGCAGGGAGAAC11750.20732428164341987No Hit
TTGAGTTCTAATTTGGTTGTGCTGCCGTCTAAGAGACTGTTTGTTATGAT11620.20503048108055652No Hit
TTGAGTACTAACGGAGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT10850.19144412390051962No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC10820.19091478530908962No Hit
TTGAGTACTAACGGGGGGGAAAATCATGGGAAAAATACTTAAAATGTCTA10760.1898561081262296No Hit
TCAAGCATGTGCACACACATGTATGCACACCCATGCATGCACACACACAA10540.18597429178907623No Hit
TCGAGCTGTTGAGGCGTGTCAGGAAATCTAGCAACATCTGTATCCTCCCC10260.1810337982690628No Hit
TGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGCT10090.17803421291762608No Hit
TCAAGGGGAAGGCTCCCCCCAGCATCTGCTCCCAACAGTTAGTCTGTTCC10070.17768132052333943No Hit
TTGAGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGGTCCTGG9960.1757404123547627No Hit
TGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAGA9870.1741523965804727No Hit
TTGAGTACTAACGGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTA9010.1589780236261458No Hit
TTGAGTGCTAACCCAAGGTTAAAAGCTCATGTGATGCTGTCCAAATCATT8390.14803835940325896No Hit
TTGAGTACTAACGTGAAATTGTTCAACTGACATGTTGGGGCCTTAGCGGC8380.14786191320611564No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT8300.14645034362896894No Hit
TTGAGTACTAACGGGTAGCGTATGAAGTAAGTGGAAAGGGGGCCTCACTC8290.1462738974318256No Hit
TTGAGTACTAACGGCTGGCTGGAGACTGGAACATATACACTGGCATACAA8150.14380365067181888No Hit
TTAAGAATCTCATTTTTCAACTATAAAATTGATATATGGAGGTTAAACTT8150.14380365067181888No Hit
TTGAGTACTAACGGAGATATTTTTGGGAACAGAGTGCTAAAACACATTAT7720.13621646419465544No Hit
TTGAGCATACACACAGTTCCCAGCACACATGCATGTGCACAAACATGTAC7420.13092307828035535No Hit
TCAAGTGTGTGACAGGCTGTGTGTGCATGTGAATGTGTGTGTTTATGTAC7390.13039373968892534No Hit
TTGAGTCCTAAACAGAGATATTTTTGGGAACAGAGTGCTAAAACACATTA7300.12880572391463532No Hit
TTGAGTACTAACGTGTTTGAAAATCTTCTAACGCAGGGCAAATAAGCCAT7240.12774704673177534No Hit
TTGAGTACTAACGGGGGGAGCAGAAGCAGAGCTGCCATCCTCAGAAAAGG6690.11804250588889184No Hit
TTGAGTACTAACGCACGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTG6650.11733672110031848No Hit
TTGAGTACTAACGCAGGACAAATAAGCCATAACAAGAAAAGTACAATGGT6410.11310201236887842No Hit
TTGAGTGAAGGGAAGATGCCTGACCACTCACACGTGTCTGTCTCTCCAGG5680.10022143997741488No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA5680.10022143997741488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG100.0072383825143.999288
TTGAGTA257100.0138.347411
TCGAGCA3550.0137.975681
CGCCTTA16450.0137.883833
CCGCCTT16400.0137.87732
TTGAGTC83700.0137.694471
AGTCTCT47700.0135.54654
TTGAGTG70750.0135.51071
GTACTAA215650.0135.218445
ACTAACG208600.0134.887187
GAGTCTC47950.0134.85173
TGAGTAC235550.0134.241672
AGTACTA221700.0134.19154
TACTAAC213800.0133.930116
CTAACGG93600.0133.845498
TACTACG700.0133.713646
CTTACAC16750.0133.253086
CCTTACA17000.0132.987585
GAGTACT228000.0132.958453
TAACGGC10700.0132.560099
Produced by FastQC (version 0.11.2)