FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_428_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_428_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183139
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC75310.23659035939052614No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG68200.21425391728102355No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC38710.12160951815173637No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT34850.10948312341999517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA82100.0118.885581
TAGTAAC85200.0114.6228562
GTAACAA87700.0111.505614
CGCACTT24550.0103.5335
TAACAAG104900.092.870545
AGTAACA105900.092.482593
GCACGCA28050.089.093862
AAGAGCG16050.087.927159
AGCACGC29700.084.40261
CTCGCTC26950.083.103373
TCGCTCT27800.080.817674
ACGCACT33000.079.2116854
ACAAGAG129600.074.888177
CGCTCTG30450.073.5408555
AAGCGGT5450.071.358952
AACAAGA137050.071.0821846
TCTGTCG32050.070.762648
CACGCAC35450.070.693593
GTCTCGC34250.066.67021
GAAGCGG16100.066.21691
Produced by FastQC (version 0.11.2)