FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_412_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_412_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855516
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC433945.072260483731456No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA386594.518793336419191No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA194202.2699750793673057No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG148381.734391875780231No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA135291.5813848016869352No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT126491.4785229031368203No Hit
TTGAGTAGCTGGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA114441.3376722352358108No Hit
TTGAGTACCACCTTCCAATCTTGGATGCCATTCTTTGATGAACAAATCCT99841.167014994459484No Hit
TTGAGTAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCGTGCCAAC89001.0403078376091155No Hit
TTGAGTAGCTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG79750.9321859556104153No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAGGAAATATTTCCAACAGGGAGA76170.8903398650638913No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTTGGTTTTTTGGAC62490.7304363682268946No Hit
TTGAGTAGCTGGCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA59520.6957204774662309No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT57490.6719921076870568No Hit
TTGAGTTGTTGCCCTTTTGTTTCTCACCACCAAAGCTAAGGAGCAATGGG56530.6607708096634077No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT54990.6427699774171377No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT54860.641250426643102No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC52500.6136647356682985No Hit
TTGAGTAGCTGGCTGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG49560.5792995104708737No Hit
TTGAGTAGCTGGCACAGAATTCAAGCCTAGATCAGCATTAAAATGCAAGG48710.5693639861791013No Hit
TTGAGTAGCTGGCGAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA46860.5477396097793612No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA45860.5360507576713937No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA45180.5281023382379757No Hit
TTGAGTCCCAGCTCTGTCACTTGCTACTATGACCTTGGATGTCATATAGC44870.5244787940845057No Hit
TTGAGTATCTATCACAGTCTTATTTGCAATTTCTACAGCTAGCTCCAATG41700.4874251329022485No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG41160.48111315276394595No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC38740.45282613066266436No Hit
TTGAGAGGGAATTAGTTGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCA37510.43844884256986427No Hit
TTGAGTAGCTGGCAAGAGCCATAGGAAATTGCCCAATATGGGTGAAAACA37230.4351759639796333No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA36460.42617554785649825No Hit
TTGAGTAGCTGGCAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACAC35730.417642685817682No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT32810.3835112376624166No Hit
TTGAGTAGCTGGCATATCCCAGAAATAGTGTTGAAGTACAATCTAATGGA30350.35475666147681634No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT29630.34634068795907963No Hit
TTGAGTAGCTGGCTAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA26790.3131443479724517No Hit
TTGAGTAGCTGGCAGAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA26700.31209235128273466No Hit
TTGAGTAGCTGGCAAATGCCATAGGAAATTGCCCAATATGGGTGAAAACA25920.30297504663851993No Hit
TTGAGTAGCTGGCGGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG25340.2961955124158987No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT25200.2945590731207833No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT24900.29105241748839294No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC23840.2786622342539473No Hit
TTGAGTAGCTGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCTTTG22670.26498627728762525No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT21690.253531202221817No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC20570.24043968786089331No Hit
TTGAGTTGATGGCCCCGACAGTAATGCATTGCTCAAAATAAAATATGGAG20370.23810191743929976No Hit
TTGAGTTTCCTTTGATTTCTCCGAATCTATGGGGGCTAATTAGGAGTGCT19540.2284001701896867No Hit
TTGAGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAGTCATCC19120.22349085230434032No Hit
TTGAGTAGCTGGCATAGGAAATTGCCCAATATGGGTGAAAACACCTTTGA18970.22173752448814515No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA18920.2211530818827468No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT18730.21893219998223293No Hit
TTGAGTAGCTGGCATAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA18660.21811398033467522No Hit
TTGAGTATCCGTGCCAACCTGCAATCATTCCTTCCCATCCTCCTTCTAGG18490.21612687547632073No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT17570.20537313153699055No Hit
TTGAGTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTGGTGA17090.19976248252516607No Hit
TTGAGTTGTTGGCTGGGGCACTCCCTGTCCAGCTTCCTTGCTGACCCCCA16730.19555449576629777No Hit
TTGAGTAGCTGGCGAGTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGCCT16670.19485316463981972No Hit
TTGAGTAGCTGGCAATAGCCATAGGAAATTGCCCAATATGGGTGAAAACA16440.19216472865498715No Hit
TTGAGTAGCTGGCACAGGAGAACATGCAAAAGCCATAGGAAATTGCCCAA15730.1838656436583302No Hit
TCAAGGGCCTTTACTTTTGCAATTTTCCCTGCTTGGAGCAATCTTCCTCC15650.18293053548969276No Hit
TTGAGTGTGGATGCTTAAAAAACACTTCCTCTAGCCGAATGGTATGGTGT15580.18211231584213505No Hit
TTGAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA15560.1818785387999757No Hit
TTGAGTAGCTGGCGCGAGTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGC15430.1803589880259399No Hit
TTGAGTCCGGGCAAGATAAACGAGGGTATGTCCACTCCGGTTCTGGGAGA15380.17977454542054153No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT15370.17965765689946184No Hit
TTGAGTAGCTGGCAATGCCACTTGGGGCCGAATAACTTTTGGCCCAGTTG14900.17416389640871707No Hit
TCAAGAAAGGGATAATGGCATTTCCTATTTCTTAATCACAGGTTTGTTAG14240.16644925401745847No Hit
TTGAGTCCTGGGGTGAGTCCCTCAGCGCGTCAGAAGGTTATAGAGCACCA14150.16539725732774138No Hit
TTGAGTAGCTGGCAAACGCCATAGGAAATTGCCCAATATGGGTGAAAACA13570.15861772310512018No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC12870.15043552662954288No Hit
TCAAGCTCCCCTCCAAGCTTCTGTCTTTAATTCGATGGTCCACCTGTATT12810.14973419550306483No Hit
TTGAGTAGCTGGCAAGGGTTTCCATGTTCCAGCAAAGGAACAGGTAGAAG12800.14961730698198514No Hit
TTGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGTATGT11700.1367595696632208No Hit
TTGAGTAGCTGGCTTCGGAGCTGCCTATGAAGACCTGAGAGTTTTGTCTG11620.13582446149458338No Hit
TTGAGTAGCTAGCATCAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGT11570.135240018889185No Hit
TCAAGGGTGTTGAAAAGTGAATTTACAGAGTTAAATTAAAGAGGGTGTGA11560.13512313036810533No Hit
TTGAGTATCAGCAGGGGAACGGTAAATGAGGGGCAAATAGCAAGTGCAAG11410.1333698025519102No Hit
TTGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCG11100.12974625839844023No Hit
TTGAGTAGCTGGCAAAAGTCATAGGAAATTGCCCAATATGGGTGAAAACA10950.12799293058224512No Hit
TTGAGTGGCAGCCAATAGAATTCTTTCCACGGCATGCGCATTTTGGATTA10810.12635649128712964No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG10550.12331738973905806No Hit
TTGAGTAGCTGGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA10190.11910940298018972No Hit
TTGAGTAGCTGGCTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCA10150.11864184889587104No Hit
TTGAGTAAGTGCAAGGGGAGCAGTAAGTGAGGGGACAGTAATCAAAAGGT9660.1129143113629669No Hit
TTGAGTAGCTGGCCAAAAATGAACAATGCTACCTTCAACTATACAAACGT9570.11186231467324984No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT9320.10894010164625793No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT9200.10753743939330182No Hit
TTGAGTGGCTCCAACATCTCCCACCAGTGCCGGCATTCCTGGGCAGGTGA9090.10625166566142538No Hit
TTGAGTAGCTGGCCATGGGAAATTGCCCAATATGGGTGAAAACACCTTTG9060.10590100009818637No Hit
TTGAGTACTCATGTGTTCTGATCACGGTGTCTATTGTGTGGCCTGTTCCC8800.1028618985501148No Hit
TTGAGTAGCTGGCTTGACAATGCCACTTGGGGCCGAATAACTTTTGGCCC8760.1023943444657961No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA100.007236315144.01739
CTAGCGT151.2637163E-4144.01739
GTCGATC100.0072375797144.00895
TGAGTCG1750.0139.951612
GCCGCCT19650.0138.992551
TTGAGTA525750.0138.383681
CCGCCTT19900.0137.55222
GAGTAGC321300.0136.488513
TGAGTAG346500.0136.459062
AGTAGCT307800.0136.211474
TTAAGGT50500.0135.564831
CGCCTTA20250.0134.79543
GAGTATC13900.0134.716063
TAAGGTC50650.0134.539172
TGAGTAT35200.0134.245032
TAGCTGG284300.0133.895616
AGCTGGC273950.0133.776677
TAGCTCG2050.0133.463846
TACTGGC11300.0133.168586
GAGTCGC1300.0132.962373
Produced by FastQC (version 0.11.2)