Basic Statistics
Measure | Value |
---|---|
Filename | H2CYMAFXY_n02_412_nextera.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2975328 |
Sequences flagged as poor quality | 0 |
Sequence length | 150 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC | 5668 | 0.19050000537755837 | No Hit |
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT | 5416 | 0.18203035093945946 | No Hit |
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA | 4827 | 0.16223421417739492 | No Hit |
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT | 4060 | 0.13645554372492713 | No Hit |
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC | 3546 | 0.11918013745039202 | No Hit |
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT | 3299 | 0.11087853171146173 | No Hit |
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG | 3090 | 0.10385409608621302 | No Hit |
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA | 2986 | 0.10035868314350552 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCACGC | 745 | 0.0 | 95.72502 | 1 |
GCACGCA | 795 | 0.0 | 89.688 | 2 |
GAAACAC | 2275 | 0.0 | 83.56756 | 4 |
ACGCACT | 890 | 0.0 | 83.341705 | 4 |
AAACACG | 2535 | 0.0 | 74.99022 | 5 |
CGGTGCA | 405 | 0.0 | 74.68967 | 2 |
CTCTACG | 30 | 0.0019875763 | 72.02218 | 2 |
CGTGCGG | 30 | 0.001987974 | 72.01855 | 3 |
CGCACTT | 960 | 0.0 | 72.00766 | 5 |
GCGGTGC | 1210 | 0.0 | 69.05883 | 1 |
AACACGA | 2180 | 0.0 | 67.70907 | 6 |
AACACGT | 640 | 0.0 | 67.50264 | 6 |
CACGCAC | 1060 | 0.0 | 67.26261 | 3 |
GTAACAA | 4400 | 0.0 | 66.94002 | 4 |
CACTCGA | 90 | 3.0013325E-10 | 64.00358 | 9 |
ACGTGCA | 985 | 0.0 | 61.419933 | 2 |
ACACGAG | 2485 | 0.0 | 60.266933 | 7 |
CGGTGCC | 465 | 0.0 | 57.307972 | 2 |
TAACAAG | 5275 | 0.0 | 55.831528 | 5 |
CTGTACG | 40 | 0.00621865 | 54.00302 | 9 |