FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_412_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_412_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975328
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC56680.19050000537755837No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT54160.18203035093945946No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA48270.16223421417739492No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT40600.13645554372492713No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC35460.11918013745039202No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT32990.11087853171146173No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30900.10385409608621302No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA29860.10035868314350552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC7450.095.725021
GCACGCA7950.089.6882
GAAACAC22750.083.567564
ACGCACT8900.083.3417054
AAACACG25350.074.990225
CGGTGCA4050.074.689672
CTCTACG300.001987576372.022182
CGTGCGG300.00198797472.018553
CGCACTT9600.072.007665
GCGGTGC12100.069.058831
AACACGA21800.067.709076
AACACGT6400.067.502646
CACGCAC10600.067.262613
GTAACAA44000.066.940024
CACTCGA903.0013325E-1064.003589
ACGTGCA9850.061.4199332
ACACGAG24850.060.2669337
CGGTGCC4650.057.3079722
TAACAAG52750.055.8315285
CTGTACG400.0062186554.003029