FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_394_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_394_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546786
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC8189214.97697453848489No Hit
TTGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA206533.777163277772291No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGT147082.689900619255065No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA137882.521644665371827No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC132362.4206910930418846No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG113962.0841791852754095No Hit
TTGAGTTACACGTGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT109882.0095613274663213No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT108591.9859689165413892No Hit
TTGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC90651.6578698064690756No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC86361.5794113236256964No Hit
TTGAGTTACACGTGCAGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC85591.565329031833295No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA83151.520704626672958No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT80081.4645583464097471No Hit
TTGAGTTACACGTTTGGGGGTTCCACTCTGACAACGAGACTCAAATGGCA65911.205407600048282No Hit
TTGAGTTACACGTGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG59021.0793985215422488No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA51290.9380269428990501No Hit
TTGAGTTACACGTCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGA47560.8698101268137809No Hit
TTGAGTTACACGTCTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTT40930.7485561078740129No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA39770.7273412267322134No Hit
TTGAGTTACACGTACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAA37980.6946044704875399No Hit
TTGAGTTACACGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAATACCC32150.5879814040593577No Hit
TTGAGTTACACGTAGACCAATATAACATAATAAGAAAATTCAATACATCA24160.4418547658498938No Hit
TTGAGTTACACGTCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAA22290.40765491435406176No Hit
TTGAGTTACACGTGCTGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC21690.39668169997037234No Hit
TGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTCC21400.3913779796849225No Hit
TTGAGTCACTGAGGATAAATCATCTGCTTATTTTAAAATGTGTAGTCCAA20630.37729568789252105No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC19140.3500455388396923No Hit
TTGAGTTACACGTTAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGA18650.34108408042634597No Hit
TTGAGTTACACGTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTTGT16610.3037751515218019No Hit
TTGAGTTACACGTGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAG16540.3024949431770382No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA15800.28896131210382126No Hit
TTGAGTTACACGTGCAGATACAGAGGTTAATCCAGGGCTGCTGGAACAGC15750.28804687757184716No Hit
TTGAGTTACACGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACA15610.2854864608823196No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT15130.27670788937536805No Hit
TTGAGTTACACGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGA15020.274696133405025No Hit
TTGAGTTACACGTCCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAG14610.26719777024283725No Hit
TTGAGTTACACGTACTTGAATATATGTTAAACATAGCTCTCTGTACCAGA13850.25329836535683065No Hit
TTGAGTTACACGTTTGGGGGTTCCACTCTGACAACGAGACTCAAAGATCG13000.23775297831327064No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT12620.23080327587026733No Hit
TTGAGTTACACGTAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAG12560.22970595443189842No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGTGTAACTCAAAGATCG12480.22824285918073983No Hit
TTGAGTTACACGTTGTGATCTATTTCTTCCTTCCCTTCTTTGACATCTCT12300.224950894865633No Hit
TTGAGTTAAACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTAT11870.2170867578906556No Hit
TTGAGTTACACGTGCCGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC11280.206296430413361No Hit
TTGAGTTACACGTGCGGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC10830.19806651962559393No Hit
TTGAGTTACACGTGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTT10740.19642053746804053No Hit
TTGAGTTACACGTGCGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGCT10520.19239702552735438No Hit
TTGAGTTACACGTGCCTCCTGTTTTGTTGTGATCTATTTCTTCCTTCCCT10430.19075104336980098No Hit
TTGAGTTACACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAA10160.18581309689714073No Hit
TTGAGTTACACGTTATCAATGCAGAAGATGCTCCAGGAGGACCCTACAAA9880.18069226351808568No Hit
TTGAGTTACACGTCTTCGAGGTTTTGCAGGTAATTCCGATACCTACACAT9280.16971904913439628No Hit
TTGAGTTACACGTGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAA8990.16441532884894638No Hit
TTGAGAATGAATGTACGTACTTGAATATATGTTAAACATAGCTCTCTGTA8980.16423244194255157No Hit
TTGAGTTACACGTTTTGAAGCAAACAACAGTAGACCAATATAACATAATA8970.16404955503615673No Hit
TTGAGTTACACGTGGAGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC8970.16404955503615673No Hit
TTGAGTCTACCAAGTGCTGGCTGCTGAATCAGGCTCAGGCACACACAGAT8750.1600260430954706No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG8740.1598431561890758No Hit
TTGAGTCTCGTTGTCAGAGTGGACCCCCCAAACGGTAATTTGGTCTTCTC8210.15015015015015015No Hit
TTGAGTTACACGTTAACCCTATTTCTCACATCAGGGGGAGTATTATTATC8080.14777262036701744No Hit
TTGAGTTACACGTGCAGAAACAGAGGTTAATCCAGGGCTGCTGGAACAGC7870.14393199533272613No Hit
TTGAGTTACACGTCTGGACGTAGCCTTGGGCAGACCAAAATGCACAGGGA7700.14082291792401413No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA7290.13332455476182636No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT6930.12674062613161274No Hit
GCCGCCTTACACTGACTTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACG6900.12619196541242825No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTCGGTTTTTTGGAC6880.1258261915996386No Hit
TTGAGTTACACGTGCAGGGACAGAGGTTAATCCAGGGCTGCTGGAACAGC6650.12161979275255767No Hit
TTGAGTTACACGTAGATGCCCCTCTCTGGCACTCACATCATACATTGTAC6530.11942514987581979No Hit
TTGAGTTACACGTGCAGCGACAGAGGTTAATCCAGGGCTGCTGGAACAGC6500.11887648915663532No Hit
TTGAGTGCCAGTCTCCGGAGAGCCCTTCCACCGAGCAGGTGCTGGGAACA6430.11759628081187157No Hit
TTGAGTTCATCCATGGCACCGCTTAGTCTTTGAAGATTCTTTACTTCCAG6380.11668184627989743No Hit
TCCAGGAGGCCCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA6220.11375565577758026No Hit
TGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAG6000.10973214383689414No Hit
TTGAGTTACACGTGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGG5990.10954925693049931No Hit
TTGAGTTACACGTGCAGGACAGAGGTTAATCCAGGGCTGCTGGAACAGCT5930.10845193549213039No Hit
TTGAGTTACACGTGCAGAAGATGCTCCAGGAGGACCCTACAAAATTGGAA5840.10680595333457696No Hit
TTGAGTGCCAGAGAGGGGCATCTACAGGGTGGTGAGCTGGACGGAAAAAT5680.10387976283225979No Hit
TTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGATACAA5490.10040491161075814No Hit
TTGAGTTACACGTGAGGCTTGGTCAGGGCCTGCAGGTTGAGGAAGCTCTG5490.10040491161075814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACTC100.0072237425144.096371
AGTTCGT151.2630087E-4144.030464
AGTGTCG100.00723363144.030464
TCGCACG100.0072356085144.017296
TCTCGTG151.2634692E-4144.017296
CGCGTCA100.0072356085144.017299
CCCGTTG100.0072375876144.004127
CGCCTTA9600.0143.293413
CCTTACA9550.0142.509255
TTAAGGC12650.0141.248611
GCCGCCT9900.0140.457571
CCGCCTT9900.0139.704242
GAGTTAC215200.0138.320713
TGAGTTA229350.0138.102422
GTTACAC207850.0138.093085
TTGAGTT242350.0138.09111
TTACACG204950.0137.974126
AGTTACA211700.0137.805244
CTTACAC9900.0137.471056
GACACGT1100.0137.458487
Produced by FastQC (version 0.11.2)