FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_394_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_394_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169882
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT93480.2949005672766368No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT66060.2083989246287401No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC64980.2049918577410768No Hit
GTATTAATCCACCTTTGTCGGCTCCATTTAAATCATTCAACCTAAAAGAG55660.17559013237716736No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA54300.17129975185196167No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA51830.16350766369221315No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT50270.15858634485447723No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC49810.15713518673565766No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC47440.14965856773217426No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA47040.14839669110711376No Hit
CCATAGCATTCTGTGAGGCTGCTTGAAAAAGACCTGGGTGTTCTTCATCC46400.146377688507017No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC46320.1461253131820049No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT41390.13057268377813433No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT39680.12517816120600073No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC38530.12155026590895182No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC37520.11836402743067409No Hit
GCTTGGAGGCCTGGGTGCTACCCACAGTGCTACATTGCCACCCAGTGCTG36320.1145783975554926No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT35750.1127802233647814No Hit
ATTCTATCCAAAGCCTCTAAAACGCAATCTAATTGCGCATAGGCACTCGG35080.11066658001780508No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT34400.10852138975520226No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA34290.1081743736833106No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA33480.10561907351756311No Hit
ATGGTAGATCCAACAAATGGACCATTACCCGAAGATAATGAGCCGAGTGC32420.1022751004611528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGT3950.0125.8340451
TAGTAAC26700.082.543132
GTAACAA25700.079.854664
GTCGTCC6350.078.252493
TAGAAAC52050.072.092122
GAAACAC44400.071.0361944
AACACGA33650.068.6858756
ACACGAG33250.068.6438147
GTAGAAA56350.066.730251
GAAGCGG14050.065.626581
AAACACG51700.060.7235375
ACGAGCC16900.058.794929
CACGAGC39100.058.373578
CGGTCCG2400.054.0001567
AGTAACA41400.053.5763553
TAACAAG39150.053.152935
CGGAGCG1057.2961484E-848.0031665
AAGCGGA13500.047.481782
ACGAGCG3500.047.315919
ACGAGCT10350.046.61039
Produced by FastQC (version 0.11.2)