FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_379_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_379_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650832
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC557578.567034196228827No Hit
TTGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCA342355.260190033679966No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC265964.086461636797208No Hit
TTGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATA218223.352939007301423No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC209803.22356614302923No Hit
TTGAGTTCCAATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC90711.3937544558349926No Hit
TTGAGTTTCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC76231.1712700051626226No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA68951.0594131818964034No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC67051.0302197802197801No Hit
TTGAGTTCCAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTC65040.9993362342355631No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA59360.9120633281707107No Hit
TTGAGTTCCAATGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCC58350.896544730437348No Hit
TTGAGTTCCAATGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCT58330.8962374314723308No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT47310.7269157017479165No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT40830.6273508370823807No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA39720.6102957445239324No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA38400.5900140128328047No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT37800.5807950438822922No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC37630.578183002679647No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA36250.5569793740934681No Hit
TTGAGTTCCAATGGGGACAAAGGGAGTGGAGGCATCAAGGGAGGATTTGT29790.45772180839294935No Hit
TTGAGTTCCAATGCACGGTAGAACAAAAATTAGACAGCTGCCTAACCTTC26050.4002569019347543No Hit
TTGAGTTCCAATGCGAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC24320.37367554146077636No Hit
TTGAGTTTGGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCC24170.3713707992231482No Hit
TTGAGTTCCAATGATGCACGATAGAACAAAAATTAGACAGCTGCCTAACC24160.3712171497406397No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA23220.35677409838483665No Hit
TTGAGTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAA22400.34417484081913613No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC21910.33664601617621753No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG21090.324046758610517No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTCGGTTTTTTGGAC20610.31667158345010693No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA20170.30991100621973106No Hit
TTGAGTTCCAATGATGGGGACAAAGGGAGTGGAGGCATCAAGGGAGGATT19280.29623620227647074No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA18400.282715047815719No Hit
TTGAGTTCCAATGCCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAG18290.281024903508125No Hit
TTGAGTTCCAATGCACGCTAGAACAAAAATTAGACAGCTGCCTAACCTTC18250.2804103055780908No Hit
TTGAGTTCCAATGCGCGATAGAACAAAAATTAGACAGCTGCCTAACCTTC18180.279334759200531No Hit
TTGAGTTCCAATGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG17820.27380337783022346No Hit
TTGAGTTCCAATGCAGAAGATGCTCCAGGAGGACCCTACAAAATTGGAAC17000.26120412026452294No Hit
TTGAGTTCCAATGGCCATGGGAAATTGCCCAATATGGGTGAAAACACCCT16920.2599749244044546No Hit
TTGAGTACTATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAA16830.25859207906187776No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT15500.2381566978882415No Hit
TTGAGTTCCAATGCAGAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC15500.2381566978882415No Hit
TTGAGTTCCAATGCTTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATT15340.23569830616810483No Hit
TTGAGTTCCAATGGCCGTAGGAAATTGCCCAATATGGGTGAAAACACCCT14740.22647933721759225No Hit
TTGAGTTCCAATGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTT14450.2220235022248445No Hit
TGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAACC14390.22110160532979325No Hit
TTGAGTTCCAATGCACAGGGAAAATACCCTCGGCAAGAGTTTCAATACTC13760.21142168793175506No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG13500.2074268013865329No Hit
TTGAGTTCCAATGGCCACAGGAAATTGCCCAATATGGGTGAAAACACCCT12880.19790053347100325No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC12810.19682498709344348No Hit
TTGAGTCCTAATGCAGCTCTTATTATGTCACAAGCTACATCACCTCCGTC12700.1951348427858495No Hit
TTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCT12560.1929837500307299No Hit
TTGAGTTCTATTTGTGAGCTTATTGTATCAGCTCTGAGATCATCCACTTT12310.18914251296801632No Hit
TTGAGTTCCAATGGCCATCGGAAATTGCCCAATATGGGTGAAAACACCCT12050.18514762642279423No Hit
TTGAGTTCCAATGTGCACAGATACTTATTTGGACACCCCCAGACCAAATG11950.18361113159770878No Hit
TTGAGTTCCAATGGCCCTGACAATAATGCATTGCTCAAAGTAAAATATGG11620.17854069867492686No Hit
GCCGCCTTACACTGACTTGAGTTTCAAAGCATCCATTCCCTATCTCTACA11080.17024362661946554No Hit
TTGAGTTCAAATGCATCCCATTGGAACATGTCCTCTTTTCCTAGAAGAAA10820.16624874007424342No Hit
TTGAGTTCCAATGATTCCAACACTTTACTCCCCTCCTTTTCAAAGCCCAA10780.16563414214420927No Hit
TTGAGTTCCAATGCACGACAGAACAAAAATTAGACAGCTGCCTAACCTTC10730.16486589473166655No Hit
TTGAGTTCCAATGCACGTTAGAACAAAAATTAGACAGCTGCCTAACCTTC10630.1633293999065811No Hit
TTGAGTCCAGGTCTCAATCAATGATAGTAGCTTGTAGGAAAATAATCAGA10510.16148560611647858No Hit
CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCT9730.14950094648081225No Hit
TTGAGTTCCAATGTGACCGGTGTAATACCACTGACAACAACACCCACCAA9530.14642795683064141No Hit
TTGAGTTTCAATGTATAGTCAGATGCATTCATTGATTCATTTATCCCATG9450.14519876097057305No Hit
TTGAGTTCCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC9280.14258671976792783No Hit
TTGAGTTCCAATGGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAAG9150.14058927649531677No Hit
TTGAGTTCCAATGTGCCTGTAGAGACAACAGGTACACAGCAAAAAGACCT9000.13828453425768863No Hit
TTGAGTTCCAATGAGGAGCTACATAGACAATATAGACCCAAAAGGAGCAA8790.1350578951250092No Hit
TTGAGGCTATGGCCCACAGATTAAGAATGGATGCACGATTAGATTATGAA8760.1345969466774836No Hit
TTGAGTTCCAATGACACCCACCAAATCTCATTTTGCAAATCTCAAAGGAA8520.13090935909727855No Hit
TTGAGTTCCAATGGTGGGCCCAACATTTACAATTTGAGAAACTTGCATAT8360.12845096737714187No Hit
TTGAGTTCCAATGCATAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC8260.12691447255205646No Hit
TTGAGTTCCAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATAT8180.1256852766919881No Hit
TTGAGTACCACCTTCCAATCTTGGATTTCATTCTTTGATGAACAAATCCT8020.12322688497185141No Hit
TTGAGTTCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC7930.12184403962927452No Hit
TTGAGTTCCAATGTGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAAGGAT7920.121690390146766No Hit
TTGAGTTCCAATGCATGATAGAACAAAAATTAGACAGCTGCCTAACCTTC7830.12030754480418909No Hit
TTGAGTTCCAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGA7550.1160053592939499No Hit
TTGAGTTCCAATGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCTTTA7530.11569806032893282No Hit
TTGAGTTCCAATGGCCTTAGGAAATTGCCCAATATGGGTGAAAACACCCT7500.11523711188140717No Hit
TTGAGTTTCAATGTCTGCAATCCCCGGATTTCTGCTTCCGATCACTTGAT7500.11523711188140717No Hit
TTGAGTTACAAAGATAGCTATCATCAGTGTTACAGCCAAACTGGAGGCAG7450.11446886446886448No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA7330.11262507067876196No Hit
TTGAGTTCCAATGTACGATAGAACAAAAATTAGACAGCTGCCTAACCTTC7310.11231777171374488No Hit
TTGAGTTCCAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCA7260.11154952430120216No Hit
TTGAGTTCCAATGCTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC7180.1103203284411338No Hit
TGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCAT7040.1081692356860142No Hit
TCGAGAGTACCACCTTCCAATCTTGGATTTCATTCTTTGATGAACAAATC6710.10309880276323229No Hit
TTGAGTTCCAATGGCCAAAGGAAATTGCCCAATATGGGTGAAAACACCCT6680.10263785431570667No Hit
TTGAGTTCCAATGCACAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC6570.10094771000811269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGT100.00723531144.021384
AGTCGCC100.00723531144.021384
AGGTCCG151.2634364E-4144.021364
CCACTAG100.007238635143.999246
TTACCCT100.007238635143.999247
CTTACAC18350.0142.037386
GCCGCCT18700.0141.732641
CCGCCTT18600.0140.924132
CGCCTTA18500.0140.90743
CCTTACA18700.0139.378945
TTACACT18600.0139.35417
TTGAGTT315450.0138.655271
GAGTCCC71500.0136.064933
TTAAGGT34300.0135.224521
AGTCCCC69100.0134.954884
AGTACTT2300.0134.628664
CAAGCCG4400.0134.201722
CCCATAG70500.0133.68448
CCAATGC84950.0133.659098
TGAGTTC251500.0133.57052
Produced by FastQC (version 0.11.2)