FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_379_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_379_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615684
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA108550.4149966127406827No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC105770.4043684175917274No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT104000.3976015451407739No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT49630.18974004505131353No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC44660.1707392789037208No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA39330.1503621997152561No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG38500.14718903353769033No Hit
CAACAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA34400.13151435723887137No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33840.12937342584195952No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31900.121956627788372No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30900.11813353600817224No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA30860.11798061233696425No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC28310.1082317282974549No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28110.10746710994141494No Hit
CACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27850.106473106078563No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27650.10570848772252306No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT27480.1050585621198891No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG27440.10490563844868112No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAA26250.10035615923024341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA35300.0132.190344
CACGCAC5850.0112.025843
CGGTGCA2100.0106.318352
GCACGCA6200.0102.224942
AGCACGC6150.0101.902491
GAAACAC19450.0101.815184
TAGAAAC22550.0100.926772
GAAGCGG6550.096.779191
AAACACG20900.094.74425
TAACAAG49950.093.412665
GGTGCAT2650.086.9634553
CGCACTT7650.085.653715
TAGTAAC56450.083.441032
ACGCACT8150.080.405034
ACAGCGT900.080.012344
AACACGT4600.079.827716
ACAAGAG58350.079.465397
ACGTGCA12200.078.515912
CAGCCTT5250.072.691365
ACCGTCA702.9704097E-972.011094
Produced by FastQC (version 0.11.2)