FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_361_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_361_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842996
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA11896314.111929356722927No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC415514.928967634484624No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT302773.5915947406630635No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA141791.6819771386815594No Hit
TTGAGTGAGTTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA125341.4868397952066201No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT119371.41602095383608No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA86571.0269325121352888No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG78970.9367778732046177No Hit
TTGAGTGAGTTGCGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCA76760.9105618531997779No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA63390.7519608633967421No Hit
TTGAGTGAGTTGCCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC59310.7035620572339607No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT56790.6736686769569488No Hit
TTGAGTGAGTTGCAAAAGATAAATCGCAATTATTCTTCATGTCTTGCTTC53180.63084522346488No Hit
TTGAGTGAGTTGCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA52190.619101395498911No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG50010.5932412490687975No Hit
TTGAGTCAGATGCATTCATTAGTTCATTTATCCCATGTAGCTCACTTGCT45710.5422327033580231No Hit
TTGAGTGAGTTGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG43380.5145931890542779No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA40260.47758233728273913No Hit
TTGAGTGAGTTGCTTCGGAGCTGCCTATGAAGACCTGAGAGTTTTGTCTG38970.4622797735695069No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA35320.418981821977803No Hit
TTGAGTGAGTTGCTGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG34550.4098477335598271No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC33580.3983411546436756No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC32350.383750338079896No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT30380.3603813066728668No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA29250.34697673535817486No Hit
TTGAGTGAGTTGCTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC28190.33440253571784445No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA26480.31411774195844344No Hit
TTGAGTCACTTGCTTTGGGTGATTCCTCATACCCCCATTGATCAAAGAGA23780.2820891202330734No Hit
TTGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTGGTGA23460.27829313543599254No Hit
TTGAGTGAGTTGCCTTCATGAAAAATACGGTGGATTAAACAAAAGCAAGC22190.2632278207725778No Hit
TTGAGTGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG20930.2482811306340718No Hit
TTGAGTGAGTTGCGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCT20910.24804388158425425No Hit
TTGAGTGAGTTGCGGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG20150.23902841769118713No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC19540.2317923216717517No Hit
TTGAGTGAGTTGCATTGACAGGCACAGAATTCAAGCCTAGATCAGCATTA18590.22052299180541782No Hit
TTGAGTATTTTGAAAGTGAAATTTATTTTATTACAAATGTTTTTCTTTTA18150.21530351270943163No Hit
TTGAGTGAGCTGCCTATGAAGACCTGAGAGTTTTGTCTGCATTAACAGGC18000.2135241448357999No Hit
TTGAGTGAGTTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCCAATG17640.20925366193908393No Hit
TTGAGTGAGTTGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA17350.20581355071672938No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT17290.20510180356727672No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT16990.2015430678200134No Hit
TTGAGTGAGTTGCAGGAGTAAAGTAATAAAAGGGGCCTTGCCTTTAATTG16420.19478146990021306No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT15840.18790124745550393No Hit
TTGAGTGAGTTGCCCGGGTTATTAGTAGAAACAACAGCATTTTGTGTCTA15830.18778262293059517No Hit
TTGAGTGAGTTGCAGGGGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG15680.1860032550569635No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT15350.18208864573497383No Hit
TTGAGTGAGTTGCATGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC14050.16666745749683273No Hit
TTGAGTGAGTTGCCTAGATCAGCATTAAAATGCAAGGGTTTCCATGTTCC14050.16666745749683273No Hit
TTGAGTGAGTTGCATTAGGATTAAAGATCAGACAAAGACAAAGATTTGGA14000.16607433487228884No Hit
TTGAGTAAGTTAAGGGTTTGGGATGGGGGCGTTATCCTGGGTTGTCCACC13070.1550422540557725No Hit
TTGAGTGAGTTGCCTATGAAGACCTGAGAGTTTTGTCTGCATTAACAGGC12700.15065314663414772No Hit
TTGAGTGAGTTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA12010.14246805441544208No Hit
TTGAGTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCA11980.14211218084071575No Hit
TTGAGTGAGTTGCATTAGCAGGCACAGAATTCAAGCCTAGATCAGCATTA11860.14068868654181038No Hit
TTGAGTGAGTTGCAAGGGTTTCCATGTTCCAGCAAAGGAACAGGTAGAAG11570.13724857531945583No Hit
TTGAGTGAGTTGCAGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC11450.13582508102055052No Hit
TTGAGTGAGTTGCCGGGTTATTAGTAGAAACAACAGCATTTTGTGTCTAA11300.13404571314691885No Hit
TTGAGTGAGTTGCTTCGGGGCTGCCTATGAAGACCTGAGAGTTTTGTCTG11170.13250359432310474No Hit
TTGAGTGAGTTGCATTTACAGGCACAGAATTCAAGCCTAGATCAGCATTA10900.12930073215056775No Hit
TTGAGTGAGTTGCTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAG10680.12669099260257463No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC10590.12562337187839562No Hit
TCAAGTCTTTGCTATTCTTACTGTATCTCTGATCAAATACACAATCATGA10540.12503024925385173No Hit
TTGAGTGAGTTGCAGATTGCCTTCATGAAAAATACGGTGGATTAAACAAA10450.12396262852967274No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA10160.12052251730731818No Hit
TTGAGTGAGTTGCATTGGGATTAAAGATCAGACAAAGACAAAGATTTGGA10140.12028526825750062No Hit
TTGAGTGAGTTGCGCGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGC10040.11909902300841285No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA9920.11767552870950752No Hit
TTGAGTTTCTCTACAGCCTTCTTAAATAAGGTCTGAGATGCTCAGTTCTT9840.1167265325102373No Hit
TTGAGTGAGTTGCAGGTGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG9620.1141167929622442No Hit
TTGAGTGAGTTGCTTTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA9560.11340504581279151No Hit
TTGAGTGAGTTGCTGGAGACTGGAACATATACACTGGCATACAATTATAT9280.11008355911534574No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG9170.1087786893413492No Hit
TTGAGTTCATCCATGGCACCACTTAGCCTTTGAAGATTCTTTACTTCTAG9090.10782969314207898No Hit
TTGAGTGAGTTGCATTACCAGGCACAGAATTCAAGCCTAGATCAGCATTA8700.10320333667063664No Hit
TGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCTT8670.10284746309591031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT100.0072335843144.035374
GTCGCTT151.2633769E-4144.026829
GTCGCGC351.2732926E-11144.026829
GTTTCGC100.0072348677144.026829
GTCGGTT100.0072374344144.009745
GTACGTT100.0072374344144.009745
TTAGTCG151.2645718E-4143.992656
GCCGCCT23200.0139.739211
CCGCCTT23500.0138.245472
TTAAGGT47100.0137.35651
CTTACAC23650.0136.077626
AGTGAGT325350.0135.712434
AGTAAAT149050.0135.19324
GTGAGTT309800.0134.875475
GTAAATT148500.0134.796985
TTGAGTG374350.0134.348741
AATTTCC147650.0134.297078
CGCCTTA24050.0134.153523
TGAGTAA172100.0133.731032
CCTTACA24100.0133.55265
Produced by FastQC (version 0.11.2)