FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_361_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_361_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699485
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA42540.1575856135522146No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC42160.15617793764366167No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT39150.1450276626838082No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT37600.13928582674102652No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT27480.10179719465009066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT6950.083.928355
AGCACGC7400.079.831761
GCACGCA7500.079.7160952
AAACACG24000.075.9132845
CACGCAC8050.075.1547243
AACACGA18350.074.55246
ACGCACT8150.074.23124
GAAACAC25150.072.4514854
ACGAGCC6650.069.306649
ACACGAG20600.066.061257
CGGTGCA2400.060.0271762
GTAACAA39350.058.3862724
GAAGCGG6200.058.099431
GCGGTGC9450.054.8901331
TAACAAG42200.054.094775
GGTGCGT3200.054.017463
ACGTGCA12850.052.6931232
CGGTGCC3850.052.387362
CACGAGC26050.051.9639638
CGGTGCG3450.050.109652
Produced by FastQC (version 0.11.2)