FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_346_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_346_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796052
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA334854.2063835025852585No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG222492.7949179199348784No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA108021.3569465311311322No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT84531.0618653052815645No Hit
TTGAGTTTCATCTGGTTTACCTTTCTGTTTTAACAGGACAGCATTGAGTT75690.9508172832930512No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT75360.9466718254586384No Hit
TCAAGCTCACTCTCCGCCACCCCTAAAGACCTCTTCCTCCCTCCTCTGAA67580.8489395165139966No Hit
TTGAGTATCCTTCAGAAGCTGAAGCCCTTTATCCCAGAGCTAAACACACA62490.7849989699165382No Hit
TTGAGTATACTTCAGGGATCCAGTGGTTCCGGAATTTAATTCTATAAAGT47680.5989558471054655No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT43980.5524764713862914No Hit
TTGAGTTTGCCTCTGTCAACAGATGGAATGGAGCCTGGGGCTGGGTGTCA43180.5424268766361997No Hit
TTAAGTCTCTGTGATAACCACGAAGGCTGTGTGAAAAAGGTAAGAGGATT39690.49858551953892455No Hit
TTGAGTATGCTTCAGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC37170.4669292960761357No Hit
TTGAGTTTGCCATTTGTATTTACTTTGTTTCAACTTAAAAAAATAAAAAC36690.46089953922608073No Hit
TTAAGAGTTAGTTACTGACAGAGTGCCGCAGCAGTCTGAACACGTTGATG34920.4386648108415028No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT33010.4146714033756589No Hit
TTGAGTATGCTTCGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGCT30960.38891931682854886No Hit
TCAAGCAAGTGAGTTTTAGGAAAACACTTTTGTTAGGAAGATCATGTGGG30030.3772366629315673No Hit
TTGAGTATGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAA29270.3676895479189801No Hit
TCAAGTATAACTGAGAGGTCACATTGGAGGCTGTGGGCGTGCCGCCTGAC28360.35625813389075084No Hit
TTGAGTAAGTTAAGGGTTTGGGATGGGGGCGTTATCCTGGGTTGTCCACC27240.34218870124062245No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT25470.3199539728560446No Hit
TTGAGTTTCATGGTGCCCCAGATAGATTATACTCAGAGACTTACTCATAG25190.3164366146935125No Hit
TTAAGTCTTTGTAGTTGGTGTGCCCCGTGCCTGAAATACACTCCCCATTT23800.29897544381522817No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA23770.2985985840120997No Hit
TTGAGTACTCTTCTTATGGGCAAAAGGTGAGATGGATACCCTTAGCAATC22730.2855341108369805No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACAACAGCATTTTGTGTCTAA22020.2766150954962741No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACACGAGCCAAAATAAAACTC21960.27586137589001725No Hit
TTGAGTATGCTTCAGAGGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC21160.26581178113992554No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA20820.26154070337113655No Hit
TCAAGATTCTCAGGTGGCCAGCTGGTTCTTCTACTGGTGTTTTCTATCCC20570.2584002050117329No Hit
TTGAGTTTGCGGATTCCAAAAGTGAGACAGTGTAAGTACCGCCAGCCTGG19970.25086300894916413No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACACGAGCTGTGGCATGGCTG18620.2339043178083844No Hit
TTGAGTATGCTTCTGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC18350.23051257958022842No Hit
TTGAGTATGCTTCGGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC17980.22586464200831102No Hit
TTGAGTTCCCTTAAAAATAAATTAGAGAACAAAATTAAGTCAGAAAAAGC17800.22360348318954038No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT17280.2170712466019808No Hit
TTAAGTTTTAGGAGTGTCAGACTCCTTCCTGGATCTGAATGCCCACCTAC16860.21179520935818266No Hit
TTGAGTCTTTGTAGTTGGTGTGCCCCGTGCCTGAAATACACTCCCCATTT16280.20450925316436613No Hit
TGGAGATGTTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCT15830.19885635611743957No Hit
TTAAGATGGAATGTTAAATACACTCTTTTATATTGGAAATAAAATGAAAA15660.1967208172330451No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC15450.194082798611146No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT15370.19307783913613685No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT15350.19282659926738455No Hit
TCGAGCATAGAGAGCAAGGGCAGAGTAGTAGGAGTCAGAGAGACTGAGCA14960.18792742182671482No Hit
TTGAGTATGCTTCGGGGGGACAATGAGACAGAGGCAGGGGCGCTGGTCTT14780.1856662630079442No Hit
TTGAGTATGCTTCTTTCACAGACTGGAGGGAAAAAGGAAACAGCTGAATA14680.18441006366418272No Hit
TTGAGTATGCTTCACAGACTGGAGGGAAAAAGGAAACAGCTGAATACAGC14450.18152080517353136No Hit
TTGAGTAGCCTCTGATCCTTTTGTTACGTGGGCATGGAAATTTAGGGTTT14210.17850592674850388No Hit
TTGAGTATGCTTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCAT14190.17825468687975157No Hit
TTAAGTTGAGAAATAAAGTTCACAGTTTCAAAAGGAAGGTATGCCATAAC14020.17611914799535708No Hit
TTGAGTATGCTTCTGAGGTGAGGACAGGAGTTGGAAAAGGGCCTGCCTGC13850.17398360911096258No Hit
TTGAGTATGCTTCTGTGGGAAGAAGAGCAGGCAGTAGCGAAGGCCTTAGC13280.16682327285152226No Hit
TTGAGTATGCTTCAGATGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC13120.16481335390150392No Hit
TTGAGTAAGCCTGGGTGCACCTTCAGAGGAAGAAATAACAGAGGCTGCAG12830.1611703758045957No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT12790.1606678960670911No Hit
TTGAGTATGCTTCTGTGACATTATGTACTGAGTAAACACTGGGAAGAGGC12470.1566480581670544No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT12430.15614557842954985No Hit
TTGAGTAGATTAATGACCAGTAAAGGCACACCCCAGATGCCCTGGCACTA12130.15237698039826544No Hit
TTGAGTGAAGAAGGCACAGGTCTTAAAATAGGTTTTCTTGCAGGGAGAAC12100.152000120595137No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA12070.15162326079200855No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC12040.15124640098888012No Hit
TTGAGTATGAATCCTGAGGGCTGATAAATCAACAAAGTGAGTCCAATAGC11460.14396044479506365No Hit
TTCAGGAGGGTAATGAGAGTTGCAGCAAGTATAAATCTTGTCACAACCCC11370.14282986538567832No Hit
TTGAGCAAGTGAAGGGAAAAACTTTGCTGCCTCTTCCAGCAGGCTCAGAA11130.1398149869606508No Hit
TTGAGTAAACAAATCTTAGTGAATTCAAGGACCTTCGAAAGAGGTTAGAG11010.13830754774813706No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACACGTGCTGTGGCATGGCTG10620.13340837030746736No Hit
TTGAGTATGCTTCTTGAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAA10570.13278027063558662No Hit
TTGAGTATGCTTCTTAAACTTCAGTAGGTAGTGGCGATTTTGCATGTGAA10310.1295141523418068No Hit
TTGAGTAAGGGTGCAGGGAGCAGCCCTGTGCATTGCCTAGGGAGGGGTTA10240.1286348128011738No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT10180.12788109319491692No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACAACAGCTACAAACTATTGA10060.12637365398240316No Hit
TTGAGTATGCTTCTGTTTCCATTAGTCAGATATTTTACATGGGCCAAAAA9520.11959017752609126No Hit
TTGAGTATGCTTCAGGAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC9370.11770587851044907No Hit
TTGAGTCTAATCACAACAGAAAGACTTTTTGAACAGGGGAAAGGTTTTTA9340.11732901870732063No Hit
GCCGCCTTACACTGACTTGTGACGACACAATGTAATGATGGCATTGAGTC9310.1169521589041922No Hit
TTGAGTATCCTTCGACCTATATCTTCTCATTTCCTCCCCTCTTTGACCAG9170.11519347982292615No Hit
TTCAGGGATCCAGTGGTTCCGGAATTTAATTCTATAAAGTGAAATTAATA8990.11293232100415551No Hit
TTGAGTATGCTTCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTG8990.11293232100415551No Hit
TTGAGTATGCTTCAGTCCCTAGGAAGGAGGCACCTTTTGCTAGTTTGTCT8700.10928934290724726No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA8700.10928934290724726No Hit
TTGAGTATGCTTCTGGAGACTGGAACATATACACTGGCATACAATTATAT8520.10702818408847663No Hit
TTGAGTATGCTTCAGGGGGACAATGAGACAGAGGCAGGGGCGCTGGTCTT8470.1064000844165959No Hit
TTAAGCAAATGGCCCTGAGGTGGAGACATGTGAGCAGAGGAGAGAGATGG8320.1045157854009537No Hit
TTGAGTAACTTGCCCTCTCAGCATAAACAACTGTAAACTGAATAAAATAT8240.10351082592594454No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT8230.1033852059915684No Hit
TTAAGCTCTATCAACTTCAAGACACTACTGTAAGTAAAATGATATCAGCC8220.10325958605719224No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA8200.10300834618843994No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACACGTGCCAAAATAAAACTC8080.1015009069759262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA403500.0138.850431
CGCCTTA22900.0137.440733
AGTATGC290850.0136.715624
CCGCCTT23000.0136.225432
GAGTATG312850.0136.19513
TATGCTT285800.0135.065616
ATGCTTC284450.0134.87957
GCTTCCG28200.0133.540479
TGAGTAT375150.0133.417912
GTATGCT299050.0130.871575
TGCTTCG48950.0130.4688
TTGAGTG63600.0129.922671
GCTTCGA11500.0129.60839
TTGAGTC50150.0129.572921
TTACACT23900.0127.447157
GCTTCGC7900.0126.6916669
TGCTTCC94250.0125.5895548
TGGCTCT45400.0125.476636
TGCTTCT100700.0124.48088
GGCTCTG45700.0124.164567
Produced by FastQC (version 0.11.2)