Basic Statistics
Measure | Value |
---|---|
Filename | H2CYMAFXY_n02_346_nextera.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3654547 |
Sequences flagged as poor quality | 0 |
Sequence length | 150 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC | 9647 | 0.26397252518574804 | No Hit |
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC | 7822 | 0.21403473535844525 | No Hit |
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC | 6741 | 0.18445514587717712 | No Hit |
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA | 6258 | 0.17123873355575944 | No Hit |
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC | 5986 | 0.1637959506335532 | No Hit |
AAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTG | 5237 | 0.143300934424978 | No Hit |
CCTCCACCAGCTTCAGCTGAAGCCTCTGAACATCTCCAGCATGGAAGAAG | 4669 | 0.12775865244037088 | No Hit |
GTAGAAACACGTGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC | 4603 | 0.12595268305483553 | No Hit |
ATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGAACACAGCTGT | 4561 | 0.12480342980949485 | No Hit |
GTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT | 3788 | 0.10365169746072496 | No Hit |
AGTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAA | 3666 | 0.1003133904147354 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTAGTAA | 5760 | 0.0 | 101.679344 | 1 |
TAGTAAC | 6385 | 0.0 | 91.36291 | 2 |
AAACACG | 6400 | 0.0 | 86.18687 | 5 |
TAACAAG | 6985 | 0.0 | 80.51502 | 5 |
AACACGA | 4370 | 0.0 | 80.40832 | 6 |
GTAACAA | 7270 | 0.0 | 78.450356 | 4 |
AGCACGC | 1850 | 0.0 | 74.76428 | 1 |
AACACGT | 2855 | 0.0 | 74.400955 | 6 |
ACGAGCC | 2855 | 0.0 | 73.399155 | 9 |
GCACGCA | 1920 | 0.0 | 72.39388 | 2 |
GTAGAAA | 12025 | 0.0 | 69.91179 | 1 |
ACACGAG | 5030 | 0.0 | 68.85378 | 7 |
CGCACTT | 1755 | 0.0 | 66.06038 | 5 |
AGTAACA | 8955 | 0.0 | 65.298996 | 3 |
ACACGTG | 3295 | 0.0 | 63.371395 | 7 |
CACGAGC | 5525 | 0.0 | 62.81444 | 8 |
GCGGTGA | 365 | 0.0 | 59.187855 | 4 |
AAGCGGT | 635 | 0.0 | 57.841858 | 2 |
TAGAAAC | 14630 | 0.0 | 56.95539 | 2 |
GAAACAC | 9810 | 0.0 | 56.376278 | 4 |