FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_346_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_346_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654547
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC96470.26397252518574804No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC78220.21403473535844525No Hit
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC67410.18445514587717712No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA62580.17123873355575944No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC59860.1637959506335532No Hit
AAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTG52370.143300934424978No Hit
CCTCCACCAGCTTCAGCTGAAGCCTCTGAACATCTCCAGCATGGAAGAAG46690.12775865244037088No Hit
GTAGAAACACGTGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC46030.12595268305483553No Hit
ATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGAACACAGCTGT45610.12480342980949485No Hit
GTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT37880.10365169746072496No Hit
AGTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAA36660.1003133904147354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA57600.0101.6793441
TAGTAAC63850.091.362912
AAACACG64000.086.186875
TAACAAG69850.080.515025
AACACGA43700.080.408326
GTAACAA72700.078.4503564
AGCACGC18500.074.764281
AACACGT28550.074.4009556
ACGAGCC28550.073.3991559
GCACGCA19200.072.393882
GTAGAAA120250.069.911791
ACACGAG50300.068.853787
CGCACTT17550.066.060385
AGTAACA89550.065.2989963
ACACGTG32950.063.3713957
CACGAGC55250.062.814448
GCGGTGA3650.059.1878554
AAGCGGT6350.057.8418582
TAGAAAC146300.056.955392
GAAACAC98100.056.3762784