FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_338_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_338_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687538
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA284224.1338806000541055No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC223203.2463660190418566No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA120441.7517577210277833No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT109981.5996206755117537No Hit
TTGAGTACTGAATGGGAGATGATGCAAAAGATAAATCGCAATTATTCTTC85911.24953093501741No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT85631.2454584328429847No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA67990.9888907958541928No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT67740.985254633198456No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT66940.973618912700098No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC65490.9525291692968243No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG52690.7663576413230978No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC49210.7157422571552409No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC45330.6593090127382051No Hit
TTGAGTTGATTGGTTGTTCTGCTTTTAGCTCTTTGAGGAATTGCCATACT44540.6478187387460765No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC43460.6321105160732934No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG41840.6085481820641186No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA41020.5966215685533018No Hit
TTGAGTACTGAATTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCCA40880.5945853174660892No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT36660.5332068918372511No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA35860.5215711713388933No Hit
TTGAGTACTGAATATCCCAGAAATAGTGTTGAAGTACAATCTAATGGACC35100.5105172368654532No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT34030.4949544606988996No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA31030.45132050883005737No Hit
TTGAGTACTATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAA30220.43953934182547No Hit
TTGAGTACTGAATCCAGGGCTGCTGGAACAGCTTGTAGTATGACTGGCCC25980.3778700231841731No Hit
TCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCT24740.3598346564117183No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC24220.35227143808778566No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC24050.3497988474818846No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAGGAAATATTTCCAACAGGGAGA23630.3436900942202467No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA23360.339763038552051No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA22380.3255092809415625No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA19940.2900203334215709No Hit
TTGAGTACTGAATTCGAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA19740.2871114032969814No Hit
TTGAGTACTGAATGCAAAAACCTGGGAAAACAGGAACAATTGTCTATCAA19440.28274800811009715No Hit
TTGAGTACTGAATGGGGGATGATGCAAAGGATAAATCGCAATTATTCTTC16630.24187753985961502No Hit
TTGAGTTCCAATACTGAAAGTTTTGGGGCTATTTACTCTGTCTCCATTGA16320.23736869816650136No Hit
TTGAGTACTGAATTTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCC16250.23635057262289505No Hit
TTGAGTATTGAAACAATCACAGTCATAGTGTTTATTTTGTTGCTTTAATA16100.23416887502945294No Hit
TTGAGTACTGAATGCCACAGGGGAGATTCCAAAGTGGAAGGAAAAAGAAT15870.23082360538617502No Hit
TTGAGTACTGAATGGGAGCAGCTCTGATGTCCATCAAGCTCCAGTTTTGG15750.22907824731142135No Hit
TTGAGTACTGAATAGCCATAGGAAATTGCCCAATATGGGTGAAAACACCT15340.2231149405560129No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA14960.21758797331929292No Hit
TTGAGTACTGAATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGG14100.20507957378355815No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC13740.1998434995592971No Hit
TTGAGTACTGAATTGCCCGATATGGGTGAAAACACCTTTGAAGCTTGCCA13370.19446197882880656No Hit
TTGAGTACTGAATTGGTGAAGCAGATTGCCTTCATGAAAAATACGGTGGA12850.18689876050487392No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT12660.18413527688651393No Hit
TTGAGTACTGAATTGCCCCATATGGGTGAAAACACCTTTGAAGCTTGCCA12490.18166268628061286No Hit
TTGAGTACTGAATCGAGGTCATCATAATCCTCTGCTGTGTCCCTCCCAAA12450.18108090025569495No Hit
TTGAGTATTGAAACAATCACAGTCATAGTGTCTATTTTGTTGATTTAATA12340.17948098868717074No Hit
TTGAGTGCTGAAGACAGGTCAAGTATAAAGAGGGCTGAGATTGGACCACT12150.17671750506881076No Hit
TTGAGTACTGAATGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA12000.17453580747536862No Hit
TTGAGTATTGAATTTAATGCGATTTTTCTACCTCCAAAACTGTTTCACCC11840.17220866337569707No Hit
TTGAGTACTGAATGATGCAAAAACCTGGGAAAACAGGAACAATTGTCTAT11330.1647908915579939No Hit
TTGAGTACTGAATTACAGGAGCAAGAAGGTAGTCAGGGATGCTTGGGAAC11060.16086383588979808No Hit
TTGAGTGTGGATGCTTAAAAAACACTTCCTCTAGCCGAATGGTATGGTGT10950.1592639243212739No Hit
TCAAGAATCATGTTCCTAGTGCCTCCATGGCATTCTGTGAGGCTGCTTGA10820.15737311974029072No Hit
TTGAGTACTGAATGCCATAGGAAATTGCCCAATATGGGTGAAAACACCTT10640.15475508262816018No Hit
TTGAGTATTGAAAGGAGGAGGGGAAAATTTCATCGAAGTAAGGAAAGGGT10590.1540278500970128No Hit
TTGAGTACTGAATATGGGTGAAAACACCTTTGAAGCTTGCCAATGGAACC10500.15271883154094756No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA10470.15228249202225913No Hit
TTGAGTACTGAATCCCCGGAAAGAGCAACTACAAGCAGTGAAGATGATGT10240.14893722237898122No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC10240.14893722237898122No Hit
TTGAGTCCATTAAACAGTAAATGGCTGTTGCTGCTGAGTGCCAAGTCTCT10170.1479190968353749No Hit
TTGAGTACTGAATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACT10150.14762820382291597No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA10040.14602829225439176No Hit
TTAAGTGTTGCATTGAAGAGATTTTATTTTCCTCTATAGGTGGTCAGGGG9430.13715605537439385No Hit
TTGAGTACTGAATTGTCGTTGATTACATGATGCAAAAACCTGGGAAAACA9430.13715605537439385No Hit
TTGAGTACTGAATAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACAC9280.13497435778095174No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT9220.1341016787435749No Hit
TTGAGTACTGAATTCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA8960.13032006958160858No Hit
TTGAGTACTGAATATGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAA8940.13002917656914964No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT8930.12988373006292017No Hit
TTGAGTCTGAAAAACCTATGGCAAGTTCTCATAGGAGAGGAAGATGTTGA8900.12944739054423174No Hit
TTGAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA8530.1240658698137412No Hit
TTGAGTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAA8300.12072060017046331No Hit
TTGAGTACTGAATGGGGACAAAGGGAGTGGAGGCATCAAGGGAGGATTTG8030.1167935445022675No Hit
TTGAGTACTGAATGCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA7900.11490273992128434No Hit
TTGAGTACTGAATGAGCCATAGGAAATTGCCCAATATGGGTGAAAACACC7860.11432095389636646No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT7760.11286648883407172No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT7700.11199380979669488No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC7690.11184836329046541No Hit
TTGAGTACTGAATGGGAGCTGATGCAAAAGATAAATCGCAATTATTCTTC7620.11083023774685909No Hit
TTGAGTACTGAATTCTAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA7370.10719407509112222No Hit
TTGAGTACTGAATCCCAGAAATAGTGTTGAAGTACAATCTAATGGACCCT7250.10544871701636854No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA7040.10239434038554959No Hit
TTGAGTACAGAATTCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA6970.10137621484194329No Hit
TTGAGTCCAGAAGTTCGAGAGCAGCCTAGGCAACAAGGCAAAACCTCGTC6940.10093987532325487No Hit
TTGAGTCATTCATCATCTTGAGTAAATTTCCTTTGACATCTGCATCACGT6890.1002126427921075No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG100.007239472143.994258
GCCGCCT26800.0141.390061
CGCCTTA26700.0140.789153
CCGCCTT26950.0140.582642
CCTTACA26450.0140.203835
CTTACAC27050.0137.0746
TTAAGGT29300.0134.9811
TAAGGTC29300.0134.223862
TTAAGGC9350.0134.061951
AGTACTG255300.0133.701864
TTGAGTA400400.0133.678831
AGCACAC12650.0133.180476
CCGTCCA5900.0133.011646
TGAGTAC294050.0132.740262
TCAAGGC4950.0132.435411
ACTGAAT248950.0132.426137
GTACTGA255950.0132.367955
AGTACGG1100.0130.932434
GAGTACT283300.0130.705983
TACTGAA256100.0130.471946
Produced by FastQC (version 0.11.2)